BMRB Entry 17523

Name: Backbone and side-chain 1H, 15N, and 13C resonance assignments of Norwalk virus protease
Published: 2011-06-08

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PDB ID: 2lnc
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17523
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Backbone and side-chain 1H, 15N, and 13C resonance assignments of Norwalk virus protease PubMed: 21647610

Deposition date: 2011-03-11 Original release date: 2011-06-08

Authors: Takahashi, Daisuke; Kim, Yunjeong; Chang, Kyeong-Ok; Anbanandam, Asokan; Prakash, Om

Citation: Takahashi, Daisuke; Kim, Yunjeong; Chang, Kyeong-Ok; Anbanandam, Asokan; Prakash, Om. "Backbone and side-chain (1)H, (15)N, and (13)C resonance assignments of Norwalk virus protease." Biomol. NMR Assignments 6, 19-21 (2012).

Assembly members:
NVpro, polymer, 188 residues, Formula weight is not available

Natural source: Common Name: Nowwalk virus Taxonomy ID: 11983 Superkingdom: Viruses Kingdom: not available Genus/species: Norovirus Nowwalk virus

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
NVpro: MHHHHHHAPPTLWSRVTKFG SGWGFWVSPTVFITTTHVVP TGVKEFFGEPLSSIAIHQAG EFTQFRFSKKMRPDLTGMVL EEGCPEGTVCSVLIKRDSGE LLPLAVRMGAIASMRIQGRL VHGQSGMLLTGANAKGMDLG TIPGDCGAPYVHKRGNDWVV CGVHAAATKSGNTVVCAVQA GEGETALE

Data sets:

assigned_chemical_shifts
- NVpro_BB_and_SC_assingnment

Data type	Count
13C chemical shifts	683
15N chemical shifts	177
1H chemical shifts	1123

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Entity Assembly ID	Entity Name	Entity ID
1	Norwalk virus protease	1

Samples:

NVpro_15N_labeled: NVpro, [U-99% 15N], 0.7-0.8 mM; sodium phosphate 20 mM; sodium chloride 100 mM; sodium azide 3 mM; D2O 5 mM

NVpro_13C15N_labeled: NVpro, [U-99% 13C; U-99% 15N], 0.7-0.8 mM; sodium phosphate 20 mM; sodium chloride 100 mM; sodium azide 3 mM; D2O, [U-99% 2H], 5 mM

NVpro_13C15N_labeled_D2O: NVpro, [U-99% 13C; U-99% 15N], 0.7-0.8 mM; sodium phosphate 20 mM; sodium chloride 100 mM; sodium azide 3 mM; D2O 5 mM

NVpro_15N_Val: NVpro, [U-15N]-Val, 0.7 mM; sodium phosphate 20 mM; sodium chloride 100 mM; sodium azide 3 mM; D2O 5 mM

NVpro_15N_Ala: NVpro, [U-15N]-Ala, 0.7 mM; sodium phosphate 20 mM; sodium chloride 100 mM; sodium azide 3 mM; D2O 5 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	NVpro_15N_labeled	isotropic	sample_conditions_1
2D 1H-15N HSQC	NVpro_13C15N_labeled	isotropic	sample_conditions_1
2D 1H-15N HSQC	NVpro_13C15N_labeled	isotropic	sample_conditions_1
3D HNCO	NVpro_13C15N_labeled	isotropic	sample_conditions_1
3D HNCO	NVpro_13C15N_labeled	isotropic	sample_conditions_1
3D HN(CA)CO	NVpro_13C15N_labeled	isotropic	sample_conditions_1
3D HNCA	NVpro_13C15N_labeled	isotropic	sample_conditions_1
3D HN(CO)CA	NVpro_13C15N_labeled	isotropic	sample_conditions_1
3D HNCA	NVpro_13C15N_labeled	isotropic	sample_conditions_1
3D HNCACB	NVpro_13C15N_labeled	isotropic	sample_conditions_1
3D CBCA(CO)NH	NVpro_13C15N_labeled	isotropic	sample_conditions_1
3D HBHA(CO)NH	NVpro_13C15N_labeled	isotropic	sample_conditions_1
3D H(CCO)NH	NVpro_13C15N_labeled	isotropic	sample_conditions_1
3D C(CO)NH	NVpro_13C15N_labeled	isotropic	sample_conditions_1
3D HNCACB	NVpro_13C15N_labeled	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	NVpro_13C15N_labeled_D2O	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	NVpro_13C15N_labeled	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	NVpro_13C15N_labeled	isotropic	sample_conditions_1
3D 1H-15N NOESY	NVpro_15N_labeled	isotropic	sample_conditions_1
3D hCCH-TOCSY	NVpro_13C15N_labeled_D2O	isotropic	sample_conditions_1
3D HCCH-TOCSY	NVpro_13C15N_labeled_D2O	isotropic	sample_conditions_1

PDB	2FYQ 2FYR 2LNC 3UR6 3UR9 4IMQ 4IMZ 4IN1 4INH 4XBB 4XBC 4XBD
GB	AAB50465 AFJ38515 AGM33207 AGM33210 AGM33213
REF	NP_056820 NP_786949
SP	Q83883

Biological Magnetic Resonance Data Bank

BMRB Entry 17523

Title: Backbone and side-chain 1H, 15N, and 13C resonance assignments of Norwalk virus protease PubMed: 21647610

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