BMRB Entry 17526
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17526
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Title: EcNusB bound to BoxA RNA PubMed: 21652641
Deposition date: 2011-03-15 Original release date: 2011-06-30
Authors: Stagno, Jason; Altieri, Amanda; Bubunenko, Mikhail; Tarasov, Sergey; Li, Jess; Court, Donald; Byrd, Andrew; Ji, Xinhua
Citation: Stagno, Jason; Altieri, Amanda; Bubunenko, Mikhail; Tarasov, Sergey; Li, Jess; Court, Donald; Byrd, R. Andrew; Ji, Xinhua. "Structural basis for RNA recognition by NusB and NusE in the initiation of transcription antitermination." Nucleic Acids Res. 39, 7803-7815 (2011).
Assembly members:
NusB, polymer, 139 residues, Formula weight is not available
BoxA, polymer, . residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
NusB: MKPAARRRAREAAVQALYSW
QLSQNDIADVEYQFLAEQDV
KDVDVLYFRELLAGVATNTA
YLDGLMKPYLSRLLEELGQV
EKAVLRIALYELSKRSDVPY
KVAINEAIELAKSFGAEDSH
KFVNGVLDKAAPVIRPNKK
BoxA: UGCUCUUUAACA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 427 |
| 15N chemical shifts | 135 |
| 1H chemical shifts | 722 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | NusB | 1 |
| 2 | BoxA RNA | 2 |
Entities:
Entity 1, NusB 139 residues - Formula weight is not available
| 1 | MET | LYS | PRO | ALA | ALA | ARG | ARG | ARG | ALA | ARG | ||||
| 2 | GLU | ALA | ALA | VAL | GLN | ALA | LEU | TYR | SER | TRP | ||||
| 3 | GLN | LEU | SER | GLN | ASN | ASP | ILE | ALA | ASP | VAL | ||||
| 4 | GLU | TYR | GLN | PHE | LEU | ALA | GLU | GLN | ASP | VAL | ||||
| 5 | LYS | ASP | VAL | ASP | VAL | LEU | TYR | PHE | ARG | GLU | ||||
| 6 | LEU | LEU | ALA | GLY | VAL | ALA | THR | ASN | THR | ALA | ||||
| 7 | TYR | LEU | ASP | GLY | LEU | MET | LYS | PRO | TYR | LEU | ||||
| 8 | SER | ARG | LEU | LEU | GLU | GLU | LEU | GLY | GLN | VAL | ||||
| 9 | GLU | LYS | ALA | VAL | LEU | ARG | ILE | ALA | LEU | TYR | ||||
| 10 | GLU | LEU | SER | LYS | ARG | SER | ASP | VAL | PRO | TYR | ||||
| 11 | LYS | VAL | ALA | ILE | ASN | GLU | ALA | ILE | GLU | LEU | ||||
| 12 | ALA | LYS | SER | PHE | GLY | ALA | GLU | ASP | SER | HIS | ||||
| 13 | LYS | PHE | VAL | ASN | GLY | VAL | LEU | ASP | LYS | ALA | ||||
| 14 | ALA | PRO | VAL | ILE | ARG | PRO | ASN | LYS | LYS |
Entity 2, BoxA RNA - Formula weight is not available
| 1 | U | G | C | U | C | U | U | U | A | A | ||||
| 2 | C | A |
Samples:
sample_1: E coli NusB, [U-100% 13C; U-100% 15N], 250 uM; boxA RNA, [U-100% 13C; U-100% 15N], 250 uM
sample_conditions_1: ionic strength: 0.02 M; pH: 6.5; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR, CCPN - chemical shift assignment
NMR spectrometers:
- Varian INOVA 600 MHz
- Varian INOVA 800 MHz
Related Database Links:
| BMRB | 4737 |
| PDB | |
| DBJ | BAB33892 BAE76196 BAG75962 BAI23787 BAI29258 |
| EMBL | CAA25289 CAA45737 CAC44764 CAP74950 CAQ30885 |
| GB | AAA24228 AAB40172 AAB95441 AAC73519 AAG54765 |
| PRF | 2111328A |
| REF | NP_308496 NP_414950 NP_706304 WP_000801121 WP_000801122 |
| SP | A7ZIG9 A7ZX68 B1J033 B1LJG6 B1XF04 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts