BMRB Entry 17574

Name: Structure of a yeast RNase III dsRBD complex with a non-canonical RNA substrate provides new insights into substrate recognition
Published: 2011-08-03

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PDB ID: 2lbs
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17574
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Structure of a yeast RNase III dsRBD complex with a non-canonical RNA substrate provides new insights into substrate recognition

Deposition date: 2011-04-06 Original release date: 2011-08-03

Authors: Wang, Zhonghua; Hartman, Elon; Roy, Kevin; Chanfreau, Guillaume; Feigon, Juli

Citation: Wang, Zhonghua; Hartman, Elon; Roy, Kevin; Chanfreau, Guillaume; Feigon, Juli. "Structure of a yeast RNase III dsRBD complex with a non-canonical RNA substrate provides new insights into substrate recognition" Not known ., .-..

Assembly members:
yeast RNase III dsRBD, polymer, 90 residues, 9822.442 Da.
non-canonical RNA substrate, polymer, 32 residues, Formula weight is not available

Natural source: Common Name: baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
yeast RNase III dsRBD: GSLDMNAKRQLYSLIGYASL RLHYVTVKKPTAVDPNSIVE CRVGDGTVLGTGVGRNIKIA GIRAAENALRDKKMLDFYAK QRAAIPRSES
non-canonical RNA substrate: GGGAUACCAUGUUCAAGUGA ACGUGGUAUCUC

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	326
15N chemical shifts	87
1H chemical shifts	547

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Entity Assembly ID	Entity Name	Entity ID
1	yeast RNase III dsRBD	1
2	non-canonical RNA substrate	2

1	GLY	SER	LEU	ASP	MET	ASN	ALA	LYS	ARG	GLN
2	LEU	TYR	SER	LEU	ILE	GLY	TYR	ALA	SER	LEU
3	ARG	LEU	HIS	TYR	VAL	THR	VAL	LYS	LYS	PRO
4	THR	ALA	VAL	ASP	PRO	ASN	SER	ILE	VAL	GLU
5	CYS	ARG	VAL	GLY	ASP	GLY	THR	VAL	LEU	GLY
6	THR	GLY	VAL	GLY	ARG	ASN	ILE	LYS	ILE	ALA
7	GLY	ILE	ARG	ALA	ALA	GLU	ASN	ALA	LEU	ARG
8	ASP	LYS	LYS	MET	LEU	ASP	PHE	TYR	ALA	LYS
9	GLN	ARG	ALA	ALA	ILE	PRO	ARG	SER	GLU	SER

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_4	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_3	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D HCCH-COSY	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_2	isotropic	sample_conditions_1
2D 1H-1H COSY	sample_4	isotropic	sample_conditions_1
2D FILTERED/EDITED NOESY	sample_4	isotropic	sample_conditions_1
2D 1H-15N HSQC IPAP	sample_1	isotropic	sample_conditions_1

BMRB	18534 18535
PDB	1T4L 1T4N 1T4O 2LBS 2LUP 2LUQ 4OOG
DBJ	GAA25690
EMBL	CAA90210 CAY82070
GB	AAB04172 AHY76694 AJP40933 AJS62105 AJS62540
REF	NP_013966
SP	Q02555
TPG	DAA10139

Biological Magnetic Resonance Data Bank

BMRB Entry 17574

Title: Structure of a yeast RNase III dsRBD complex with a non-canonical RNA substrate provides new insights into substrate recognition

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	GLY	SER	LEU	ASP	MET	ASN	ALA	LYS	ARG	GLN
2	LEU	TYR	SER	LEU	ILE	GLY	TYR	ALA	SER	LEU
3	ARG	LEU	HIS	TYR	VAL	THR	VAL	LYS	LYS	PRO
4	THR	ALA	VAL	ASP	PRO	ASN	SER	ILE	VAL	GLU
5	CYS	ARG	VAL	GLY	ASP	GLY	THR	VAL	LEU	GLY
6	THR	GLY	VAL	GLY	ARG	ASN	ILE	LYS	ILE	ALA
7	GLY	ILE	ARG	ALA	ALA	GLU	ASN	ALA	LEU	ARG
8	ASP	LYS	LYS	MET	LEU	ASP	PHE	TYR	ALA	LYS
9	GLN	ARG	ALA	ALA	ILE	PRO	ARG	SER	GLU	SER

1	GLY	SER	LEU	ASP	MET	ASN	ALA	LYS	ARG	GLN
2	LEU	TYR	SER	LEU	ILE	GLY	TYR	ALA	SER	LEU
3	ARG	LEU	HIS	TYR	VAL	THR	VAL	LYS	LYS	PRO
4	THR	ALA	VAL	ASP	PRO	ASN	SER	ILE	VAL	GLU
5	CYS	ARG	VAL	GLY	ASP	GLY	THR	VAL	LEU	GLY
6	THR	GLY	VAL	GLY	ARG	ASN	ILE	LYS	ILE	ALA
7	GLY	ILE	ARG	ALA	ALA	GLU	ASN	ALA	LEU	ARG
8	ASP	LYS	LYS	MET	LEU	ASP	PHE	TYR	ALA	LYS
9	GLN	ARG	ALA	ALA	ILE	PRO	ARG	SER	GLU	SER

1	GLY	SER	LEU	ASP	MET	ASN	ALA	LYS	ARG	GLN
2	LEU	TYR	SER	LEU	ILE	GLY	TYR	ALA	SER	LEU
3	ARG	LEU	HIS	TYR	VAL	THR	VAL	LYS	LYS	PRO
4	THR	ALA	VAL	ASP	PRO	ASN	SER	ILE	VAL	GLU
5	CYS	ARG	VAL	GLY	ASP	GLY	THR	VAL	LEU	GLY
6	THR	GLY	VAL	GLY	ARG	ASN	ILE	LYS	ILE	ALA
7	GLY	ILE	ARG	ALA	ALA	GLU	ASN	ALA	LEU	ARG
8	ASP	LYS	LYS	MET	LEU	ASP	PHE	TYR	ALA	LYS
9	GLN	ARG	ALA	ALA	ILE	PRO	ARG	SER	GLU	SER