BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17578

Title: Solution structure of the S. cerevisiae H/ACA RNP protein Nhp2p-S82W mutant   PubMed: 21708174

Deposition date: 2011-04-07 Original release date: 2011-06-30

Authors: Koo, Bon-Kyung; Park, Chin-Ju; Fernandez, Cesar; Chim, Nicholas; Ding, Yi; Chanfreau, Guillaume; Feigon, Juli

Citation: Koo, Bon-Kyung; Park, Chin-Ju; Fernandez, Cesar; Chim, Nicholas; Ding, Yi; Chanfreau, Guillaume; Feigon, Juli. "Structure of H/ACA RNP protein Nhp2p reveals cis/trans isomerization of a conserved proline at the RNA and Nop10 binding interface."  J. Mol. Biol. 411, 927-942 (2011).

Assembly members:
Nhp2p-S82W, polymer, 121 residues, 13306.792 Da.

Natural source:   Common Name: baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Fungi

Experimental source:   Production method: recombinant technology   Host organism: Saccharomyces cerevisiae

Entity Sequences (FASTA):
Nhp2p-S82W: SKKLNKKVLKTVKKASKAKN VKRGVKEVVKALRKGEKGLV VIAGDIWPADVISHIPVLCE DHSVPYIFIPSKQDLGAAGA TKRPTSVVFIVPGSNKKKDG KNKEEEYKESFNEVVKEVQA L

Data sets:
Data typeCount
13C chemical shifts351
15N chemical shifts114
1H chemical shifts643

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Related Database Links:

BMRB 17579
PDB 2LBW 2LBX
DBJ GAA22045
EMBL CAA40885 CAA67483 CAA98786 CAY79077
GB AHY74809 AJP37550 AJU57662 AJU58365 AJU59054
REF NP_010073
SP P32495
TPG DAA11656

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