BMRB Entry 17584
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR17584
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Title: 1H 13C 15N resonance assignment of CD4+ T cell-derived peptide Thp5 PubMed: 22130674
Deposition date: 2011-04-11 Original release date: 2011-12-02
Authors: PANDEY, NISHANT; BHAVESH, NEEL; DAS, GOBARDHAN
Citation: Khan, Mohd Moin; Chatterjee, Samit; Dwivedi, Ved Prakash; Pandey, Nishant Kumar; Singh, Yogesh; Tousif, Sultan; Bhavesh, Neel Sarovar; Van Kaer, Luc; Das, Jyoti; Das, Gobardhan. "CD4+ T Cell-derived Novel Peptide Thp5 Induces Interleukin-4 Production in CD4+ T Cells to Direct T Helper 2 Cell Differentiation." J. Biol. Chem. 287, 2830-2835 (2012).
Assembly members:
Thp5, polymer, 22 residues, Formula weight is not available
Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Thp5: WRPYLQTEYYDVMTVISPPE
FG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 58 |
| 15N chemical shifts | 19 |
| 1H chemical shifts | 109 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Thp5 | 1 |
Entities:
Entity 1, Thp5 22 residues - Formula weight is not available
| 1 | TRP | ARG | PRO | TYR | LEU | GLN | THR | GLU | TYR | TYR | ||||
| 2 | ASP | VAL | MET | THR | VAL | ILE | SER | PRO | PRO | GLU | ||||
| 3 | PHE | GLY |
Samples:
sample_1: Thp5 800 uM; sodium chloride 150 mM; sodium phosphate 50 mM; D2O, [U-100% 2H], 5%; H2O 95%
sample_conditions_1: ionic strength: 150 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA, Keller and Wuthrich - chemical shift assignment, data analysis, peak picking
TOPSPIN, Bruker Biospin - collection
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
NMR spectrometers:
- Bruker Avance 500 MHz
- Bruker Avance 700 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts