BMRB Entry 17610
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PDB ID: 2n46
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17610
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Title: Solution structure of GppNHp-bound H-RasT35S mutant protein. PubMed: 21930707
Deposition date: 2011-04-28 Original release date: 2012-05-09
Authors: Araki, Mitsugu
Citation: Araki, Mitsugu; Shima, Fumi; Yoshikawa, Yoko; Muraoka, Shin; Ijiri, Yuichi; Nagahara, Yuka; Shirono, Tomoya; Kataoka, Tohru; Tamura, Atsuo. "Solution structure of the state 1 conformer of GTP-bound H-Ras protein and distinct dynamic properties between the state 1 and state 2 conformers." J. Biol. Chem. 286, 39644-39653 (2011).
Assembly members:
GppNHp-bound H-RasT35S mutant protein, polymer, 172 residues, 18861.303 Da.
PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER, non-polymer, 522.196 Da.
MG, non-polymer, 24.305 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
GppNHp-bound H-RasT35S mutant protein: GPLGSDMTEYKLVVVGAGGV
GKSALTIQLIQNHFVDEYDP
SIEDSYRKQVVIDGETCLLD
ILDTAGQEEYSAMRDQYMRT
GEGFLCVFAINNTKSFEDIH
QYREQIKRVKDSDDVPMVLV
GNKCDLAARTVESRQAQDLA
RSYGIPYIETSAKTRQGVED
AFYTLVREIRQH
- assigned_chemical_shifts
- heteronucl_NOEs
- heteronucl_T1_relaxation
- heteronucl_T2_relaxation
| Data type | Count |
| 13C chemical shifts | 719 |
| 15N chemical shifts | 184 |
| 1H chemical shifts | 1177 |
| T1 relaxation values | 130 |
| T2 relaxation values | 130 |
| heteronuclear NOE values | 130 |
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