BMRB Entry 17627
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17627
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Title: 1H, 13C, and 15N chemical shift assignments for apo actinin-2 C-terminal EF-hand (Act2-EF34)
Deposition date: 2011-05-05 Original release date: 2011-06-03
Authors: Au, Yunghan; Beck, Moriah; Campbell, Sharon; Pastore, Annalisa
Citation: Au, Yunghan; Pastore, Annalisa. "The Interaction of alpha-Actinin-2 with ZASP and Titin" Not known ., .-. (2004).
Assembly members:
Act2-EF34, polymer, 75 residues, 8071.05 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Act2-EF34: GAMADTDTAEQVIASFRILA
SDKPYILAEELRRELPPDQA
QYCIKRMPAYSGPGSVPGAL
DYAAFSSALYGESDL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 135 |
| 15N chemical shifts | 67 |
| 1H chemical shifts | 276 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Ef-Hands 3,4 From Alpha-Actinin | 1 |
Entities:
Entity 1, Ef-Hands 3,4 From Alpha-Actinin 75 residues - 8071.05 Da.
N-terminal residue of natural sequence replaced by M N-terminal GA left after cleavage from fusion protein
| 1 | GLY | ALA | MET | ALA | ASP | THR | ASP | THR | ALA | GLU | ||||
| 2 | GLN | VAL | ILE | ALA | SER | PHE | ARG | ILE | LEU | ALA | ||||
| 3 | SER | ASP | LYS | PRO | TYR | ILE | LEU | ALA | GLU | GLU | ||||
| 4 | LEU | ARG | ARG | GLU | LEU | PRO | PRO | ASP | GLN | ALA | ||||
| 5 | GLN | TYR | CYS | ILE | LYS | ARG | MET | PRO | ALA | TYR | ||||
| 6 | SER | GLY | PRO | GLY | SER | VAL | PRO | GLY | ALA | LEU | ||||
| 7 | ASP | TYR | ALA | ALA | PHE | SER | SER | ALA | LEU | TYR | ||||
| 8 | GLY | GLU | SER | ASP | LEU |
Samples:
sample_1: Act2-EF34, [U-100% 13C; U-100% 15N], 0.5 – 1 mM; D2O, [U-2H], 10%; sodium phosphate 20 mM; sodium azide 0.02 mM; H2O 90%
sample_2: Act2-EF34, [U-100% 15N], 0.5 – 1 mM; D2O, [U-2H], 10%; sodium phosphate 20 mM; sodium azide 0.02 mM; H2O 90%
sample_conditions_1: ionic strength: 20 mM; pH: 6.6; pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNHB | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - peak picking, processing
XEASY, Bartels et al. - data analysis
NMR spectrometers:
- Varian Unity 500 MHz
- Varian Unity 600 MHz
- Varian INOVA 600 MHz
- Varian INOVA 800 MHz
Related Database Links:
| BMRB | 17626 4453 4454 |
| PDB | |
| DBJ | BAB22865 BAD92758 BAG37672 BAH11921 BAH12587 |
| EMBL | CAB61269 |
| GB | AAA51583 AAF76325 AAH47901 AAH51770 AAH89579 |
| REF | NP_001029807 NP_001094 NP_001163796 NP_001230595 NP_001265272 |
| SP | P35609 Q3ZC55 Q9JI91 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts