BMRB Entry 17711

Name: Solution structure of the core SMN-Gemin2 complex
Published: 2012-06-05

Click here to enlarge.

PDB ID: 2leh
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17711
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title: Solution structure of the core SMN-Gemin2 complex PubMed: 22607171

Deposition date: 2011-06-15 Original release date: 2012-06-05

Authors: Sarachan, Kathryn; Valentine, Kathleen; Gupta, Kushol; Moorman, Veronica; Gledhill, John; Bernens, Matthew; Tommos, Cecilia; Wand, A.; Van Duyne, Gregory

Citation: Sarachan, Kathryn; Valentine, Kathleen; Gupta, Kushol; Moorman, Veronica; Gledhill Jr, John; Bernens, Matthew; Tommos, Cecilia; Wand, A. Joshua; Van Duyne, Gregory. "Solution structure of the core SMN-Gemin2 complex." Biochem J. 445, 361-370 (2012).

Assembly members:
gemin2, polymer, 189 residues, 21609.596 Da.
smn, polymer, 26 residues, 2863.100 Da.

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
gemin2: GYSPTLQWQQQQVAQFSTVR QNVNKHRSHWKSQQLDSNVT MPKSEDEEGWKKFCLGEKLC ADGAVGPATNESPGIDYVQI GFPPLLSIVSRMNQATVTSV LEYLSNWFGERDFTPELGRW LYALLACLEKPLLPEAHSLI RQLARRCSEVRLLVDSKDDE RVPALNLLICLVSRYFDQRD LADEPSLEY
smn: GQSDDSDIWDDTALIKAYDK AVASFK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	914
15N chemical shifts	206
1H chemical shifts	1425

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Entity Assembly ID	Entity Name	Entity ID
1	gemin2	1
2	smn	2

Samples:

sample_1: gemin2, [U-99% 13C; U-99% 15N], 1.0 mM; smn, [U-99% 13C; U-99% 15N], 1.0 mM; sodium chloride 50 mM; DTT 50 mM; EDTA 50 uM; sodium azide 200 nM; sodium phosphate 34.25 mM; potassium phosphate 15.75 mM; H2O 95%; D2O 5%

sample_2: gemin2, [U-99% 13C; U-99% 15N], 1.0 mM; smn, [U-99% 13C; U-99% 15N], 1.0 mM; sodium chloride 50 mM; DTT, [U-98% 2H], 50 mM; EDTA 50 uM; sodium azide 200 nM; sodium phosphate 34.25 mM; potassium phosphate 15.75 mM; D2O 100%

sample_3: gemin2, [U-10% 13C; U-99% 15N], 1.0 mM; smn, [U-10% 13C; U-99% 15N], 1.0 mM; sodium chloride 50 mM; DTT 50 mM; EDTA 50 uM; sodium azide 200 nM; sodium phosphate 34.25 mM; potassium phosphate 15.75 mM; H2O 95%; D2O 5%

sample_4: gemin2, [U-98% 15N]-Leu, 1.0 mM; smn 1.0 mM; sodium chloride 50 mM; DTT 50 mM; EDTA 50 uM; sodium azide 200 nM; sodium phosphate 34.25 mM; potassium phosphate 15.75 mM; H2O 95%; D2O 5%

sample_5: gemin2 1.0 mM; smn, [U-99% 15N], 1.0 mM; sodium chloride 50 mM; DTT 50 mM; EDTA 50 uM; sodium azide 200 nM; sodium phosphate 34.25 mM; potassium phosphate 15.75 mM; H2O 95%; D2O 5%

sample_6: gemin2, [U-99% 15N], 1.0 mM; smn, [U-99% 15N], 1.0 mM; sodium chloride 50 mM; DTT 50 mM; EDTA 50 uM; sodium azide 200 nM; sodium phosphate 34.25 mM; potassium phosphate 15.75 mM; bacteriophage Pf1 strain LP11-92 12 mg/ml; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_2	isotropic	sample_conditions_1
3D HCCH-TOCSY aromatic	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_4	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_5	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_3	isotropic	sample_conditions_1
4D HNCH-NOESY-HSQC	sample_1	isotropic	sample_conditions_1
4D HCCH-NOESY-HMQC	sample_2	isotropic	sample_conditions_1
2D 1H-15N HSQC IPAP	sample_6	isotropic	sample_conditions_1

PDB	2LEH 3S6N 2LEH 3S6N
DBJ	BAB03509 BAG36665 BAD51989 BAE02007 BAE26061 BAE39854 BAF82358
EMBL	CAC16117 CAA73356 CAB41938 CAH89989 CDQ72355
GB	AAB82297 AAI04969 AIC50129 EAW65825 EAW65829 AAA64505 AAA66242 AAB58318 AAB96377 AAC01747
REF	NP_001009183 NP_003607 XP_001092707 XP_001145975 XP_002807275 NP_000335 NP_001003226 NP_001009328 NP_001124207 NP_001124942
SP	O14893 O02771 O35876 P97801 Q16637 Q4R4F8
PRF	2208336A

Biological Magnetic Resonance Data Bank

BMRB Entry 17711

Title: Solution structure of the core SMN-Gemin2 complex PubMed: 22607171

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links: