BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 17726

Title: conotoxin pc16a   PubMed: 22079220

Deposition date: 2011-06-22 Original release date: 2012-01-18

Authors: Dyubankova, Natalia; Lescrinier, Eveline; Van Der Haegen, Annelies; Peigneur, Steve; Tytgat, Jan

Citation: Van Der Haegen, Annelies; Peigneur, Steve; Dyubankova, Natalia; Moller, Carolina; Mari, Frank; Diego-Garcia, Elia; Naude, Ryno; Lescrinier, Eveline; Herdewijn, Piet; Tytgat, Jan. "Pc16a, the first characterized peptide from Conus pictus venom, shows a novel disulfide connectivity."  Peptides 34, 106-113 (2012).

Assembly members:
conotoxin_pc16a, polymer, 11 residues, 1258.5 Da.

Natural source:   Common Name: Conus pictus   Taxonomy ID: 1042615   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Conus pictus

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
conotoxin_pc16a: SCSCKRNFLCC

Data sets:
Data typeCount
13C chemical shifts33
1H chemical shifts65

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1conotoxin_pc16a1

Entities:

Entity 1, conotoxin_pc16a 11 residues - 1258.5 Da.

C-terminal amidation Disulfide bonds between Cys2/Cys10 and Cys4/Cys11

1   SERCYSSERCYSLYSARGASNPHELEUCYS
2   CYS

Samples:

sample_1: conotoxin pc16a 5 mg; H2O 90%; D2O 10%

sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

UNP P86942