BMRB Entry 17744
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17744
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Title: 3D ssNMR structure of membrane embedded KcsA-Kv1.3 channel (Inactivated state) PubMed: 23882077
Deposition date: 2011-08-11 Original release date: 2012-07-11
Authors: Nand, D.; Ader, C.; Prokofyev, A.; Hornig, S.; A Bonvin, M.; Killian, A.; Becker, S.; Pongs, O.; Baldus, M.
Citation: van der Cruijsen, Elwin; Nand, Deepak; Weingarth, Markus; Prokofyev, Alexander; Hornig, Sonke; Cukkemane, Abhishek Arun; Bonvin, Alexandre M J J; Becker, Stefan; Hulse, Raymond; Perozo, Eduardo; Pongs, Olaf; Baldus, Marc. "Importance of lipid-pore loop interface for potassium channel structure and function." Proc. Natl. Acad. Sci. U.S.A. 110, 13008-13013 (2013).
Assembly members:
KcsA-Kv1.3_(Inactivated_state), polymer, 94 residues, 9775.335 Da.
Natural source: Common Name: Streptomyces lividans Taxonomy ID: 1916 Superkingdom: Bacteria Kingdom: not available Genus/species: Streptomyces lividans
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
KcsA-Kv1.3_(Inactivated_state): SALHWRAAGAATVLLVIVLL
AGSYLAVLAEADDPTSGFSS
IPDALWWSVETATTVGYGDL
YPVTLWGRCVAVVVMVAGIT
SFGLVTAALATWFV
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 250 |
| 15N chemical shifts | 39 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | KcsA-Kv1.3 (Inactivated state) | 1 |
Entities:
Entity 1, KcsA-Kv1.3 (Inactivated state) 94 residues - 9775.335 Da.
| 1 | SER | ALA | LEU | HIS | TRP | ARG | ALA | ALA | GLY | ALA | ||||
| 2 | ALA | THR | VAL | LEU | LEU | VAL | ILE | VAL | LEU | LEU | ||||
| 3 | ALA | GLY | SER | TYR | LEU | ALA | VAL | LEU | ALA | GLU | ||||
| 4 | ALA | ASP | ASP | PRO | THR | SER | GLY | PHE | SER | SER | ||||
| 5 | ILE | PRO | ASP | ALA | LEU | TRP | TRP | SER | VAL | GLU | ||||
| 6 | THR | ALA | THR | THR | VAL | GLY | TYR | GLY | ASP | LEU | ||||
| 7 | TYR | PRO | VAL | THR | LEU | TRP | GLY | ARG | CYS | VAL | ||||
| 8 | ALA | VAL | VAL | VAL | MET | VAL | ALA | GLY | ILE | THR | ||||
| 9 | SER | PHE | GLY | LEU | VAL | THR | ALA | ALA | LEU | ALA | ||||
| 10 | THR | TRP | PHE | VAL |
Samples:
sample_1: KcsA-Kv1.3 (Inactivated state), [U-100% 13C; U-100% 15N], 5 mM; H2O 100%
sample_conditions_1: pH: 4.0; temperature: 243 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D_13C-13C_CHHC | sample_1 | solid | sample_conditions_1 |
Software:
CNS, Brunger A. T. et.al. - structure solution
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz