BMRB Entry 17783

Assembly:

Entities:

Entity 1, DamX SPOR domain 106 residues - 11876.272 Da.

first residue number is 323 residues 323-337 constitute an added affinity tag and linker the actual SPOR domain starts at residue 344

Samples:

15N_DamX: DamX SPOR domain polypeptide, [U-99% 15N], 0.7 mM; H2O 90%; D2O, [U-99% 2H], 10%; potassium phosphate 50 mM; potassium chloride 50 mM

13C-15N_DamX_in_D2O: DamX SPOR domain polypeptide, [U-99% 13C; U-99% 15N], 0.7 mM; D2O, [U-99% 2H], 100%; potassium phosphate 50 mM; potassium chloride 50 mM

13C-15N_DamX: DamX SPOR domain polypeptide, [U-99% 13C; U-99% 15N], 0.7 mM; H2O 90%; D2O, [U-99% 2H], 10%; potassium phosphate 50 mM; potassium chloride 50 mM

15N_DamX_aligned: DamX SPOR domain polypeptide, [U-99% 15N], 0.7 mM; H2O 90%; D2O, [U-99% 2H], 10%; potassium phosphate 50 mM; potassium chloride 50 mM; PEG(C12E5):n-hexanol 5%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - peak picking, processing

VNMRJ v2.1B, Varian - collection

CCPN_Analysis v1.0.15, CCPN - chemical shift assignment, data analysis, peak picking

TALOS, Cornilescu, Delaglio and Bax - data analysis

CurveFit, Palmer - data analysis

X-PLOR NIH v2.23, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

ProcheckNMR v3.5.4, Laskowski and MacArthur - structure analysis

NMR spectrometers:

• Varian INOVA 600 MHz