BMRB Entry 17819
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17819
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Title: Backbone 1H, 13C, 15N assignment of Hck kinase regulatory segment complex with full length Nef PubMed: 21365684
Deposition date: 2011-07-29 Original release date: 2014-04-23
Authors: Jung, Jinwon; Byeon, In-Ja; Ahn, Jinwoo; Gronenborn, Angela
Citation: Jung, Jinwon; Byeon, In-Ja; Ahn, Jinwoo; Gronenborn, Angela. "Structure, dynamics, and Hck interaction of full-length HIV-1 Nef." Proteins 79, 1609-1622 (2011).
Assembly members:
Hck32L, polymer, 193 residues, Formula weight is not available
Nef, polymer, 217 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Hck32L: GIREAGSEDIIVVALYDYEA
IHHEDLSFQKGDQMVVLEES
GEWWKARSLATRKEGYIPSN
YVARVDSLETEEWFFKGISR
KDAERQLLAPGNMLGSFMIR
DSETTKGSYSLSVRDYDPRQ
GDTVKHYKIRTLDNGGFYIS
PRSTFSTLQELVDHYKKGND
GLCQKLSVPCMSSKPQKPWE
KDAWELEHHHHHH
Nef: MEGKWSKRSVSGWPAVRERM
RRAEPAAEGVGAVSRDLEKH
GAITSSNTAATNAACAWLEA
QEEEEVGFPVRPQVPLRPMT
YKAAVDLSHFLKEKGGLEGL
IYSQKRQDILDLWVYHTQGY
FPDWQNYTPGPGIRYPLTFG
WCFKLVPVEPEKVEEANEGE
NNCLLHPMSQHGMDDPEKEV
LVWKFDSKLAFHHMARELHP
EYYKDCAAALEHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 259 |
| 15N chemical shifts | 146 |
| 1H chemical shifts | 146 |
Additional metadata:
Related Database Links:
| UNP | P08631 |
| BMRB | 17740 17922 |
| PDB | 1AD5 1QCF 2C0I 2C0O 2C0T 2HCK 3HCK 3NHN 3VRY 3VRZ 3VS0 3VS1 3VS2 3VS3 3VS4 3VS5 3VS6 3VS7 4LUD 4LUE |
| DBJ | BAB15482 BAF82585 BAF83617 BAG60878 |
| EMBL | CAC44031 |
| GB | AAA52643 AAA52644 AAH14435 AAH94847 AAI08931 AAA03700 |
| REF | NP_001165600 NP_001165601 NP_001165602 NP_001165603 NP_001165604 |
| SP | P08631 Q95M30 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts