BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17828

Title: Ubiquitin-like domain from HOIL-1   PubMed: 22517668

Deposition date: 2011-08-03 Original release date: 2012-04-30

Authors: Beasley, Steven; Shaw, Gary; Beasley, Steven

Citation: Beasley, Steven; Safadi, Susan; Barber, Kathryn; Shaw, Gary. "Solution structure of the E3 ligase HOIL-1 Ubl domain."  Protein Sci. 21, 1085-1092 (2012).

Assembly members:
HOIL-1_Ubl, polymer, 90 residues, 10353.854 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
HOIL-1_Ubl: MPTQDIRLWVSVEDAQMHTV TIWLTVRPDMTVASLKDMVF LDYGFPPVLQQWVIGQRLAR DQETLHSHGVRQNGDSAYLY LLSARNTSLN

Data sets:
Data typeCount
13C chemical shifts391
15N chemical shifts90
1H chemical shifts633

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HOIL-1_Ubl1

Entities:

Entity 1, HOIL-1_Ubl 90 residues - 10353.854 Da.

1   METPROTHRGLNASPILEARGLEUTRPVAL
2   SERVALGLUASPALAGLNMETHISTHRVAL
3   THRILETRPLEUTHRVALARGPROASPMET
4   THRVALALASERLEULYSASPMETVALPHE
5   LEUASPTYRGLYPHEPROPROVALLEUGLN
6   GLNTRPVALILEGLYGLNARGLEUALAARG
7   ASPGLNGLUTHRLEUHISSERHISGLYVAL
8   ARGGLNASNGLYASPSERALATYRLEUTYR
9   LEULEUSERALAARGASNTHRSERLEUASN

Samples:

sample_1: HOIL-1 Ubl, [U-15N], 0.2 mM; potassium phosphate 10 mM; EDTA 1 mM; potassium chloride 50 mM; DSS 30 uM; H2O 90%; D2O 10%

sample_2: HOIL-1 Ubl, [U-13C; U-15N], 0.2 mM; potassium phosphate 10 mM; EDTA 1 mM; potassium chloride 50 mM; DSS 30 uM; H2O 90%; D2O 10%

sample_3: HOIL-1 Ubl, [U-13C; U-15N], 0.2 mM; potassium phosphate 10 mM; EDTA 1 mM; potassium chloride 50 mM; DSS 30 uM; D2O 100%

sample_conditions_1: ionic strength: 61 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HN(CA)COsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D C(CO)NHsample_2isotropicsample_conditions_1
3D H(CCO)NHsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_3isotropicsample_conditions_1
3D HCCH-COSY aromaticsample_3isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_3isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_3isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_3isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_3isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe v5.0, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw v5.0, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

X-PLOR NIH v2.11.0, Schwieters, Kuszewski, Tjandra and Clore - refinement

TALOS vtalosplus, Cornilescu, Delaglio and Bax - geometry optimization

NMRView v8.0.rc47, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

Procheck, Laskowski, MacArthur, Smith, Jones, Hutchinson, Morris, Moss and Tho - structure verification

VNMRJ v2.1B, Varian - collection

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 17959
PDB
DBJ BAC75409 BAG59927 BAG64417
GB AAD00162 AAH15219 AIC59659 EAX10673 EAX10675
REF NP_001253226 NP_006453 NP_112506 XP_001152050 XP_002747461
SP Q9BYM8

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts