BMRB Entry 17858

Name: RNA-binding zinc finger protein
Published: 2012-06-05

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PDB ID: 2lhn
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17858
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: RNA-binding zinc finger protein PubMed: 22560733

Deposition date: 2011-08-12 Original release date: 2012-06-05

Authors: Brockmann, Christoph; Neuhaus, David; Stewart, Murray

Citation: Brockmann, Christoph; Soucek, Sharon; Kuhlmann, Sonja; Mills-Lujan, Katherine; Kelly, Seth; Yang, Ji-Chun; Iglesias, Nahid; Stutz, Francoise; Corbett, Anita; Neuhaus, David; Stewart, Murray. "Structural Basis for Polyadenosine-RNA Binding by Nab2 Zn Fingers and Its Function in mRNA Nuclear Export." Structure 20, 1007-1018 (2012).

Assembly members:
NAB2_404-483, polymer, 80 residues, 9227.831 Da.
ZN, non-polymer, 65.409 Da.

Natural source: Common Name: baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
NAB2_404-483: GPLGSEKSLEQCKFGTHCTN KRCKYRHARSHIMCREGANC TRIDCLFGHPINEDCRFGVN CKNIYCLFRHPPGRVLPEKK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	268
15N chemical shifts	80
1H chemical shifts	530

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Entity Assembly ID	Entity Name	Entity ID
1	NAB2_404-483	1
2	Zinc ions 1	2
3	Zinc ions 2	2
4	Zinc ions 3	2

1	GLY	PRO	LEU	GLY	SER	GLU	LYS	SER	LEU	GLU
2	GLN	CYS	LYS	PHE	GLY	THR	HIS	CYS	THR	ASN
3	LYS	ARG	CYS	LYS	TYR	ARG	HIS	ALA	ARG	SER
4	HIS	ILE	MET	CYS	ARG	GLU	GLY	ALA	ASN	CYS
5	THR	ARG	ILE	ASP	CYS	LEU	PHE	GLY	HIS	PRO
6	ILE	ASN	GLU	ASP	CYS	ARG	PHE	GLY	VAL	ASN
7	CYS	LYS	ASN	ILE	TYR	CYS	LEU	PHE	ARG	HIS
8	PRO	PRO	GLY	ARG	VAL	LEU	PRO	GLU	LYS	LYS

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC lomg-range optimised	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D H[C]CH-TOCSY	sample_1	isotropic	sample_conditions_1
3D [H]CCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY (50ms mixing)	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY (120 ms mixing)	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic (150 ms mixing)	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic (150 ms mixing)	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC IPAP	sample_2	isotropic	sample_conditions_1
3D HNCO IPAP	sample_2	isotropic	sample_conditions_1

Biological Magnetic Resonance Data Bank

BMRB Entry 17858

Title: RNA-binding zinc finger protein PubMed: 22560733

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	GLY	PRO	LEU	GLY	SER	GLU	LYS	SER	LEU	GLU
2	GLN	CYS	LYS	PHE	GLY	THR	HIS	CYS	THR	ASN
3	LYS	ARG	CYS	LYS	TYR	ARG	HIS	ALA	ARG	SER
4	HIS	ILE	MET	CYS	ARG	GLU	GLY	ALA	ASN	CYS
5	THR	ARG	ILE	ASP	CYS	LEU	PHE	GLY	HIS	PRO
6	ILE	ASN	GLU	ASP	CYS	ARG	PHE	GLY	VAL	ASN
7	CYS	LYS	ASN	ILE	TYR	CYS	LEU	PHE	ARG	HIS
8	PRO	PRO	GLY	ARG	VAL	LEU	PRO	GLU	LYS	LYS

1	GLY	PRO	LEU	GLY	SER	GLU	LYS	SER	LEU	GLU
2	GLN	CYS	LYS	PHE	GLY	THR	HIS	CYS	THR	ASN
3	LYS	ARG	CYS	LYS	TYR	ARG	HIS	ALA	ARG	SER
4	HIS	ILE	MET	CYS	ARG	GLU	GLY	ALA	ASN	CYS
5	THR	ARG	ILE	ASP	CYS	LEU	PHE	GLY	HIS	PRO
6	ILE	ASN	GLU	ASP	CYS	ARG	PHE	GLY	VAL	ASN
7	CYS	LYS	ASN	ILE	TYR	CYS	LEU	PHE	ARG	HIS
8	PRO	PRO	GLY	ARG	VAL	LEU	PRO	GLU	LYS	LYS

1	GLY	PRO	LEU	GLY	SER	GLU	LYS	SER	LEU	GLU
2	GLN	CYS	LYS	PHE	GLY	THR	HIS	CYS	THR	ASN
3	LYS	ARG	CYS	LYS	TYR	ARG	HIS	ALA	ARG	SER
4	HIS	ILE	MET	CYS	ARG	GLU	GLY	ALA	ASN	CYS
5	THR	ARG	ILE	ASP	CYS	LEU	PHE	GLY	HIS	PRO
6	ILE	ASN	GLU	ASP	CYS	ARG	PHE	GLY	VAL	ASN
7	CYS	LYS	ASN	ILE	TYR	CYS	LEU	PHE	ARG	HIS
8	PRO	PRO	GLY	ARG	VAL	LEU	PRO	GLU	LYS	LYS