BMRB Entry 17862

Assembly:

Entities:

Entity 1, Staphylococcus aureus IsdH linker domain 78 residues - 9280.425 Da.

Residue 1 represents a non-native residue left after cleaving of the affinity tag

Samples:

sample_1: IsdH linker, [U-100% 15N], 1.1 mM; potassium phosphate 20 mM; potassium chloride 50 mM; AEBSF protease inhibitor 100 uM; sodium azide 0.01%; D2O 7%; H2O 93%

sample_2: IsdH linker, [U-100% 13C; U-100% 15N], 1.3 mM; potassium phosphate 20 mM; potassium chloride 50 mM; AEBSF protease inhibitor 100 uM; sodium azide 0.01%; D2O 7%; H2O 93%

sample_3: IsdH linker, [U-100% 13C; U-100% 15N], 1.3 mM; potassium phosphate 20 mM; potassium chloride 50 mM; AEBSF protease inhibitor 100 uM; sodium azide 0.01%; D2O 100%

sample_conditions_1: ionic strength: 140 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Software:

PIPP, Garrett - peak picking

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

UNIO, Herrmann, Guntert and Wuthrich - chemical shift assignment

ProcheckNMR, Laskowski and MacArthur - geometry optimization

TALOS, Cornilescu, Delaglio and Bax - geometry optimization

xwinnmr, Bruker Biospin - collection

CARA, Keller - chemical shift assignment

NMR spectrometers:

• Bruker Avance 500 MHz
• Bruker Avance 600 MHz
• Bruker Avance 800 MHz