BMRB Entry 17928

Assembly:

Entities:

Entity 1, AM2-BM2_1 35 residues - 3244.864 Da.

residues 18-37 from M2 of influenza A residues 38-52 correspond to residues 20-34 from BM2 of influenza B

Samples:

sample_1: AM2-BM2, [U-100% 13C; U-100% 15N; U-80% 2H], 1.2 mM; DHPC 300 mM; sodium phosphate 40 mM; H2O 95%; D2O 5%

sample_2: influenza AM2-BM2 chimeric channel, [U-100% 13C; U-100% 15N], 1.4 mM; DHPC 300 mM; sodium phosphate 40 mM; H2O 95%; D2O 5%

sample_3: influenza AM2-BM2 chimeric channel, [U-100% 15N; U-99% 2H], 0.8 mM; influenza AM2-BM2 chimeric channel, [U-15% 13C], 0.8 mM; DHPC 300 mM; sodium phosphate 40 mM; H2O 95%; D2O 5%

sample_4: influenza AM2-BM2 chimeric channel, [U-100% 13C; U-100% 15N; U-80% 2H], 0.5 mM; DHPC 300 mM; sodium phosphate 40 mM; DNA nanotube 5.4 mg; H2O 95%; D2O 5%

sample_5: influenza AM2-BM2 chimeric channel, [U-15% 13C], 1.5 mM; DHPC 300 mM; sodium phosphate 40 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 100 mM; pH: 7.5; pressure: 1 atm; temperature: 303 K

Experiments:

Software:

xwinnmr, Bruker Biospin - collection

CARA, Keller and Wuthrich - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

• Bruker DMX 600 MHz
• Bruker Avance 800 MHz