BMRB Entry 17943
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17943
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Title: 1H, 13C and 15N NMR assignment of CGC-19, a single domain proteic constituent of a non ribosomal peptide synthetase. PubMed: 22419055
Deposition date: 2011-09-15 Original release date: 2012-03-23
Authors: Nogaret, Sophie; Guittet, Eric; Birlirakis, Nicolas
Citation: Nogaret, Sophie; Guittet, Eric; Birlirakis, Nicolas. "(1)H, (13)C and (15)N NMR assignment of CGC-19, a single domain proteic constituent of a non ribosomal peptide synthetase." Biomol. NMR Assignments 7, 1-4 (2013).
Assembly members:
CGC-19, polymer, 127 residues, 13790 Da.
Natural source: Common Name: Streptomyces ambofaciens Taxonomy ID: 1889 Superkingdom: Bacteria Kingdom: not available Genus/species: Streptomyces ambofaciens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
CGC-19: GIDPFTMTNHADNPTTHVWA
TLADREAEHLTGDAEAAAEV
VRGIWAEVLEADAESIDVHR
GDFFELGGYSLLALQAIGRI
LTEYGVGEVESVEWEGELLN
RLFENATPMGQAEFLAEKGY
GRPNETP
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 540 |
| 15N chemical shifts | 135 |
| 1H chemical shifts | 829 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CGC-19 major form | 1 |
| 2 | CGC-19 minor form | 1 |
Entities:
Entity 1, CGC-19 major form 127 residues - 13790 Da.
Residues 1-6 originate from a non-native extended affinity tag
| 1 | GLY | ILE | ASP | PRO | PHE | THR | MET | THR | ASN | HIS | ||||
| 2 | ALA | ASP | ASN | PRO | THR | THR | HIS | VAL | TRP | ALA | ||||
| 3 | THR | LEU | ALA | ASP | ARG | GLU | ALA | GLU | HIS | LEU | ||||
| 4 | THR | GLY | ASP | ALA | GLU | ALA | ALA | ALA | GLU | VAL | ||||
| 5 | VAL | ARG | GLY | ILE | TRP | ALA | GLU | VAL | LEU | GLU | ||||
| 6 | ALA | ASP | ALA | GLU | SER | ILE | ASP | VAL | HIS | ARG | ||||
| 7 | GLY | ASP | PHE | PHE | GLU | LEU | GLY | GLY | TYR | SER | ||||
| 8 | LEU | LEU | ALA | LEU | GLN | ALA | ILE | GLY | ARG | ILE | ||||
| 9 | LEU | THR | GLU | TYR | GLY | VAL | GLY | GLU | VAL | GLU | ||||
| 10 | SER | VAL | GLU | TRP | GLU | GLY | GLU | LEU | LEU | ASN | ||||
| 11 | ARG | LEU | PHE | GLU | ASN | ALA | THR | PRO | MET | GLY | ||||
| 12 | GLN | ALA | GLU | PHE | LEU | ALA | GLU | LYS | GLY | TYR | ||||
| 13 | GLY | ARG | PRO | ASN | GLU | THR | PRO |
Samples:
sample_CGC-19: CGC-19, [U-13C; U-15N], 0.5 mM
sample_CGC-19a: CGC-19, [U-13C; U-15N], 0.5 mM
CGC-19_sample_conditions: ionic strength: 90 mM; pH: 5.6; pressure: 1 atm; temperature: 298 K
CGC-19a_sample_conditions: ionic strength: 90 mM; pH: 5.6; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_CGC-19 | isotropic | CGC-19_sample_conditions |
| 2D 1H-13C HSQC | sample_CGC-19a | isotropic | CGC-19a_sample_conditions |
| 3D HNCO | sample_CGC-19 | isotropic | CGC-19_sample_conditions |
| 3D HN(CA)CO | sample_CGC-19 | isotropic | CGC-19_sample_conditions |
| 3D HNCA | sample_CGC-19 | isotropic | CGC-19_sample_conditions |
| 3D HN(CO)CA | sample_CGC-19 | isotropic | CGC-19_sample_conditions |
| 3D HNCACB | sample_CGC-19 | isotropic | CGC-19_sample_conditions |
| 3D CBCA(CO)NH | sample_CGC-19 | isotropic | CGC-19_sample_conditions |
| 3D HNHA | sample_CGC-19 | isotropic | CGC-19_sample_conditions |
| 3D HBHA(CO)NH | sample_CGC-19 | isotropic | CGC-19_sample_conditions |
| 3D H(CCO)NH | sample_CGC-19 | isotropic | CGC-19_sample_conditions |
| 3D (H)C(CO)NH | sample_CGC-19 | isotropic | CGC-19_sample_conditions |
| 3D HCCH-TOCSY | sample_CGC-19a | isotropic | CGC-19a_sample_conditions |
| 3D 1H-15N NOESY | sample_CGC-19 | isotropic | CGC-19_sample_conditions |
| 3D 1H-13C NOESY aliphatic | sample_CGC-19a | isotropic | CGC-19a_sample_conditions |
Software:
SPARKY v3.114, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker Avance III 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts