BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18008

Title: TBA   PubMed: 22101937

Deposition date: 2011-10-18 Original release date: 2011-11-02

Authors: Tripsianes, Konstantinos; Madl, T.; Machyna, M.; Fessas, D.; Englbrecht, C.; Neugebauer, U.; Sattler, M.

Citation: Tripsianes, Konstantinos; Madl, T.; Machyna, M.; Fessas, D.; Englbrecht, C.; Fischer, U.; Neugebauer, K.; Sattler, M.. "Structural basis for dimethylarginine recognition by the Tudor domains of human SMN and SPF30 proteins"  Nat. Struct. Mol. Biol. 18, 1414-1420 (2011).

Assembly members:
A, polymer, 64 residues, 6921.46 Da.
DA2, non-polymer, 202.254 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: ESCHERICHIA COLI

Entity Sequences (FASTA):
A: ASTQPTHSWKVGDKCMAVWS EDGQCYEAEIEEIDEENGTA AITFAGYGNAEVTPLLNLKP VEEG

Data sets:
Data typeCount
13C chemical shifts200
15N chemical shifts67
1H chemical shifts435

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1protein1
2DA22

Entities:

Entity 1, protein 64 residues - 6921.46 Da.

1   ALASERTHRGLNPROTHRHISSERTRPLYS
2   VALGLYASPLYSCYSMETALAVALTRPSER
3   GLUASPGLYGLNCYSTYRGLUALAGLUILE
4   GLUGLUILEASPGLUGLUASNGLYTHRALA
5   ALAILETHRPHEALAGLYTYRGLYASNALA
6   GLUVALTHRPROLEULEUASNLEULYSPRO
7   VALGLUGLUGLY

Entity 2, DA2 - C8 H18 N4 O2 - 202.254 Da.

1   DA2

Samples:

sample_new_1: SPF30, [U-13C; U-15N], 4 mM; DA2 18 mM; H2O 93%; D2O 7%; phosphate 20 mM; NaCl 50 mM

sample_conditions_new_1: ionic strength: 50.000 mM; pH: 6.5; pressure: 1.000 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N-EDITED 3D NOESY F1 15N/13C FILTEREDsample_new_1solutionsample_conditions_new_1
15N-EDITED 3D NOESYsample_new_1solutionsample_conditions_new_1
13C-EDITED 3D NOESY (ALIPHATICSS) F1 15N/13C FILTEREDsample_new_1solutionsample_conditions_new_1
13C-EDITED 3D NOESY (ALIPHATICS)sample_new_1solutionsample_conditions_new_1
13C-EDITED 3D NOESY (AROMATICS) F1 15N/13C FILTEREDsample_new_1solutionsample_conditions_new_1
13C-EDITED 3D NOESY (AROMATICS)sample_new_1solutionsample_conditions_new_1

Software:

AutoDep v4.3, AutoDep - chemical shift assignment

CNS vany, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment

CYANA vany, Guntert, Mumenthaler and Wuthrich - chemical shift assignment

ANALYSIS v2.1, CCPN - chemical shift assignment

TALOS vany, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

  • BRUKER AVANCE 750 MHz

Related Database Links:

UNP SPF30_HUMAN
BMRB 18006
PDB
DBJ BAC37414 BAC41064 BAG36724
EMBL CAH93075
GB AAC64086 AAC84148 AAH11234 AAH58779 AAH97986
REF NP_001020571 NP_001030311 NP_001030414 NP_001126821 NP_001159781
SP O75940 Q3T045 Q4QQU6 Q5R591 Q8BGT7
TPG DAA14726

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts