BMRB Entry 18015
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PDB ID: 2lkx
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18015
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Title: Chemical shift assignments for the homeodomain of Pitx2 in complex with a TAATCC DNA binding site
Deposition date: 2011-10-21 Original release date: 2011-11-28
Authors: Baird-Titus, Jamie; Doerdelmann, Thomas; Chaney, Beth; Clark-Baldwin, Kimber; Dave, Vrushank; Ma, Jun
Citation: Chaney, Beth; Clark-Baldwin, Kimber; Dave, Vrushank; Ma, Jun; Rance, Mark. "Solution structure of the K50 class homeodomain PITX2 bound to DNA and implications for mutations that cause Rieger syndrome" Biochemistry 44, 7497-7511 (2005).
Assembly members:
PITX2, polymer, 68 residues, 8486.608 Da.
DNA_1, polymer, 11 residues, 3229.152 Da.
DNA_2, polymer, 11 residues, 3478.311 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
PITX2: GSQRRQRTHFTSQQLQELEA
TFQRNRYPDMSTREEIAVWT
NLTEARVRVWFKNRRAKWRK
REEFIVTD
DNA_1: CTCTAATCCCC
DNA_2: GGGGATTAGAG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 225 |
| 15N chemical shifts | 89 |
| 1H chemical shifts | 519 |
Additional metadata:
Related Database Links:
| BMRB | 17145 17147 17452 |
| PDB | 2L7F 2L7M 2LKX |
| DBJ | BAA75247 BAA75248 BAB69053 BAD12775 BAF84280 |
| EMBL | CAA06696 CAA06697 CAA09455 CAB52283 CAB52284 |
| GB | AAB38505 AAB38864 AAB87380 AAC16257 AAC24502 |
| REF | NP_000316 NP_001007500 NP_001017227 NP_001035436 NP_001035967 |
| SP | O35160 O75364 O93385 P81062 P97474 |
| TPG | DAA14908 DAA28868 |
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