BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18025

Title: hUBR2 ubr-box assigments

Deposition date: 2011-10-26 Original release date: 2011-11-29

Authors: Matta-Camacho, Edna; Kozlov, Guennadi; Gehring, Kalle

Citation: Matta-Camacho, Edna; Kozlov, Guennadi; Gehring, Kalle. "1H and 15N Assigned Chemical Shifts for hUBR2 ubr-box"  Not known ., .-..

Assembly members:
ubr2-box, polymer, 75 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
ubr2-box: GPLGSLCGRVFKVGEPTYSC RDCAVDPTCVLCMECFLGSI HRDHRYRMTTSGGGGFCDCG DTEAWKEGPYCQKHE

Data sets:
Data typeCount
15N chemical shifts64
1H chemical shifts64

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hUBR2 ubr-box1

Entities:

Entity 1, hUBR2 ubr-box 75 residues - Formula weight is not available

1   GLYPROLEUGLYSERLEUCYSGLYARGVAL
2   PHELYSVALGLYGLUPROTHRTYRSERCYS
3   ARGASPCYSALAVALASPPROTHRCYSVAL
4   LEUCYSMETGLUCYSPHELEUGLYSERILE
5   HISARGASPHISARGTYRARGMETTHRTHR
6   SERGLYGLYGLYGLYPHECYSASPCYSGLY
7   ASPTHRGLUALATRPLYSGLUGLYPROTYR
8   CYSGLNLYSHISGLU

Samples:

sample_1: ubr2-box, [U-15N], 1 mM; zinc 10 uM; sodium chloride 10 mM; TRIS 20 mM; beta-mercaptoethanol 2 mM

sample_conditions_1: ionic strength: 0.02 M; pH: 7.6; pressure: 1 atm; temperature: 301 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, BRUKER - collection, processing

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAE25301 BAE27917 BAJ06628
GB AAH24217 AAH64512 AAH75642 AAI56046 AAL32101
REF NP_001170845 NP_001171542 NP_001171730 NP_001179567 NP_056070
SP Q6WKZ8 Q8IWV8
TPG DAA16585

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts