BMRB Entry 18121

Name: 1H, 13C, and 15N chemical shift assignments for CdnLNt from Myxoccoccus xanthus
Published: 2012-03-09

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PDB ID: 2lt4
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18121
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: 1H, 13C, and 15N chemical shift assignments for CdnLNt from Myxoccoccus xanthus PubMed: 22392343

Deposition date: 2011-12-07 Original release date: 2012-03-09

Authors: Jimenez, M. Angeles; Padmanabhan, S.

Citation: Mirassou, Yasmina; Elias-Arnanz, Montserrat; Padmanabhan, S.; Jimenez, M. Angeles. "(1)H, (13)C and (15)N assignments of CdnL, an essential protein in Myxococcus xanthus." Biomol. NMR Assignments 7, 51-55 (2013).

Assembly members:
CdnL_N-terminal_domain, polymer, 71 residues, 7776.9 Da.

Natural source: Common Name: Myxoccoccus xanthus Taxonomy ID: 34 Superkingdom: Bacteria Kingdom: not available Genus/species: Myxoccoccus xanthus

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
CdnL_N-terminal_domain: AGHMQTSFKTGDKAVYPGQG VGEVMGIEHTEVAGQRQSFY VLRILENGMRIMIPINKVGS VGLREIISEED

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
- assigned_chem_shift_list_2

Data type	Count
13C chemical shifts	431
15N chemical shifts	138
1H chemical shifts	628

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Entity Assembly ID	Entity Name	Entity ID
1	CdnLNt	1

Samples:

sample_1: CdnL N-terminal domain 1 mM; sodium phosphate 50 mM; sodium chloride 100 mM; sodium azide 0.05%; DSS 0.5 mM

sample_2: CdnL N-terminal domain 1 mM; sodium phosphate 50 mM; sodium chloride 100 mM; sodium azide 0.05%; DSS 0.5 mM

sample_3: CdnL N-terminal domain, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 50 mM; sodium chloride 100 mM; sodium azide 0.05%; DSS 0.5 mM

sample_4: CdnL N-terminal domain, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 50 mM; sodium chloride 100 mM; sodium azide 0.05%; DSS 0.5 mM

sample_5: CdnL N-terminal domain, [U-100% 13C; U-100% 15N], 0.5 mM; sodium phosphate 50 mM; sodium chloride 100 mM; sodium azide 0.05%; DSS 0.25 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 0.15 M; pH: 7.0; pressure: 1 atm; temperature: 288 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H COSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H COSY	sample_2	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_2	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_3	isotropic	sample_conditions_1
3D HNCO	sample_3	isotropic	sample_conditions_1
3D HNCA	sample_3	isotropic	sample_conditions_1
3D HNCACB	sample_3	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_3	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_3	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_4	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_4	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_4	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_5	isotropic	sample_conditions_1
3D HNCA	sample_5	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_5	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_5	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_3	isotropic	sample_conditions_2
3D HNCO	sample_3	isotropic	sample_conditions_2
3D HNCA	sample_3	isotropic	sample_conditions_2
3D HNCACB	sample_3	isotropic	sample_conditions_2
3D CBCA(CO)NH	sample_3	isotropic	sample_conditions_2
3D HBHA(CO)NH	sample_3	isotropic	sample_conditions_2
2D 1H-13C HSQC aromatic	sample_2	isotropic	sample_conditions_1

BMRB	18151
PDB	2LT4 2LWJ
GB	ABF89955 ADO71151 AEI66063 AFE08291 AGC44335
REF	WP_002617724 WP_002625147 WP_002639129 WP_011552697 WP_013939202

Biological Magnetic Resonance Data Bank

BMRB Entry 18121

Title: 1H, 13C, and 15N chemical shift assignments for CdnLNt from Myxoccoccus xanthus PubMed: 22392343

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