BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18123

Title: Resonance assignments of the 56 kDa chimeric avidin in the biotin-bound and free forms   PubMed: 22392339

Deposition date: 2011-12-08 Original release date: 2012-03-09

Authors: Tossavainen, Helena; Helppolainen, Satu; Maatta, Juha; Pihlajamaa, Tero; Hytonen, Vesa; Kulomaa, Markku; Permi, Perttu

Citation: Tossavainen, Helena; Helppolainen, Satu; Maatta, Juha; Pihlajamaa, Tero; Hytonen, Vesa; Kulomaa, Markku; Permi, Perttu. "Resonance assignments of the 56kDa chimeric avidin in the biotin-bound and free forms."  Biomol. NMR Assignments 7, 35-38 (2013).

Assembly members:
avidin, polymer, 129 residues, Formula weight is not available

Natural source:   Common Name: Chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
avidin: QTVARKCSLTGKWTNDLGSN MTIGAVNSRGEFTGTYITAV ADNPGNITLSPLLGIQHKRA SQPTFGFTVNWKFSESTTVF TGQCFIDRNGKEVLKTMWLL RSSVNDIGDDWKATRVGYNI FTRLRTQKE

Data sets:
Data typeCount
13C chemical shifts212
15N chemical shifts118
1H chemical shifts122

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1avidin1

Entities:

Entity 1, avidin 129 residues - Formula weight is not available

1   GLNTHRVALALAARGLYSCYSSERLEUTHR
2   GLYLYSTRPTHRASNASPLEUGLYSERASN
3   METTHRILEGLYALAVALASNSERARGGLY
4   GLUPHETHRGLYTHRTYRILETHRALAVAL
5   ALAASPASNPROGLYASNILETHRLEUSER
6   PROLEULEUGLYILEGLNHISLYSARGALA
7   SERGLNPROTHRPHEGLYPHETHRVALASN
8   TRPLYSPHESERGLUSERTHRTHRVALPHE
9   THRGLYGLNCYSPHEILEASPARGASNGLY
10   LYSGLUVALLEULYSTHRMETTRPLEULEU
11   ARGSERSERVALASNASPILEGLYASPASP
12   TRPLYSALATHRARGVALGLYTYRASNILE
13   PHETHRARGLEUARGTHRGLNLYSGLU

Samples:

sample_1: avidin, [U-13C; U-15N], 0.7 – 1.2 mM; d-biotin0.7 – 1.2 mM; H2O 93%; D2O 7%

sample_2: avidin, [U-13C; U-15N], 0.7 – 1.2 mM; H2O 93%; D2O 7%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 333 K

sample_conditions_2: pH: 6.5; pressure: 1 atm; temperature: 343 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

VNMR, Varian - collection, processing

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Varian Unity INOVA 800 MHz

Related Database Links:

BMRB 18125
PDB

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts