BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18205

Title: PDZ Domain of CAL (Cystic Fibrosis Transmembrane Regulator-Associated Ligand)   PubMed: 16331976

Deposition date: 2012-01-20 Original release date: 2012-03-02

Authors: Piserchio, Andrea; Fellows, Abigail; Maden, Dean; Mierke, Dale

Citation: Piserchio, Andrea; Fellows, Abigail; Madden, Dean; Mierke, Dale. "Association of the cystic fibrosis transmembrane regulator with CAL: structural features and molecular dynamics."  Biochemistry 44, 16158-16166 (2005).

Assembly members:
PDZ Domain of CAL, polymer, 112 residues, 9256.696 Da.
C-terminus of the CFTR, polymer, 8 residues, 990.055 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
PDZ Domain of CAL: HHHHHHHHHHSSGHIEGRHM ENLYFQGIRKVLLLKEDHEG LGISITGGKEHGVPILISEI HPGQPADRCGGLHVGDAILA VNGVNLRDTKHKEAVTILSQ QRGEIEFEVVYV
C-terminus of the CFTR: EEVQDTRL

Data sets:
Data typeCount
13C chemical shifts222
15N chemical shifts79

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1PDZ Domain of CAL1
2C-terminus of the CFTR2

Entities:

Entity 1, PDZ Domain of CAL 112 residues - 9256.696 Da.

Residues 1-10 represent a polyhistidine purification tag. Residues 11-27 represent a cloning linker. Residues 28-112 represent the PDZ domain of the CAL protein (residues 278-362 in the full-length sequence).

1   HISHISHISHISHISHISHISHISHISHIS
2   SERSERGLYHISILEGLUGLYARGHISMET
3   GLUASNLEUTYRPHEGLNGLYILEARGLYS
4   VALLEULEULEULYSGLUASPHISGLUGLY
5   LEUGLYILESERILETHRGLYGLYLYSGLU
6   HISGLYVALPROILELEUILESERGLUILE
7   HISPROGLYGLNPROALAASPARGCYSGLY
8   GLYLEUHISVALGLYASPALAILELEUALA
9   VALASNGLYVALASNLEUARGASPTHRLYS
10   HISLYSGLUALAVALTHRILELEUSERGLN
11   GLNARGGLYGLUILEGLUPHEGLUVALVAL
12   TYRVAL

Entity 2, C-terminus of the CFTR 8 residues - 990.055 Da.

residues 1-8 represent the C-terminus of the CFTR protein (residues 1473-1480 in the full-length sequence)

1   GLUGLUVALGLNASPTHRARGLEU

Samples:

sample_1: PDZ Domain of CAL, [U-100% 13C; U-100% 15N], 1.0 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 1.0 M; pH: 6.8; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

UNP Q9HD26-2 P13569-1
PDB
DBJ BAB69946 BAC26958 BAC27058 BAC27951
EMBL CAH90325
GB AAG00571 AAG00572 AAH09553 AAH51171 AAI68160
PRF 1611455A
REF NP_001017408 NP_001101101 NP_001125151 NP_001186201 NP_001193086
SP Q5RD32 Q8BH60 Q9HD26
TPG DAA26316 DAA26317