BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18242

Title: Backbone and sidechain 1H, 15N and 13C assignments of NLRP10 (PYNOD) pyrin domain   PubMed: 22618865

Deposition date: 2012-02-07 Original release date: 2012-06-05

Authors: Chang, Chung-I; Chang, Chi-Fon; Su, Ming-Yuan

Citation: Su, Ming-Yuan; Chang, Chung-I; Chang, Chi-Fon. "1H, 13C and 15N resonance assignments of the pyrin domain from human PYNOD."  Biomol. NMR Assignments 7, 141-143 (2013).

Assembly members:
PYNOD_PYD, polymer, 102 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
PYNOD_PYD: GAMAMAKARKPREALLWALS DLEENDFKKLKFYLRDMTLS EGQPPLARGELEGLIPVDLA ELLISKYGEKEAVKVVLKGL KVMNLLELVDQLSHICLHDY RE

Data sets:
Data typeCount
13C chemical shifts428
15N chemical shifts93
1H chemical shifts719

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PYNOD PYD1

Entities:

Entity 1, PYNOD PYD 102 residues - Formula weight is not available

1   GLYALAMETALAMETALALYSALAARGLYS
2   PROARGGLUALALEULEUTRPALALEUSER
3   ASPLEUGLUGLUASNASPPHELYSLYSLEU
4   LYSPHETYRLEUARGASPMETTHRLEUSER
5   GLUGLYGLNPROPROLEUALAARGGLYGLU
6   LEUGLUGLYLEUILEPROVALASPLEUALA
7   GLULEULEUILESERLYSTYRGLYGLULYS
8   GLUALAVALLYSVALVALLEULYSGLYLEU
9   LYSVALMETASNLEULEUGLULEUVALASP
10   GLNLEUSERHISILECYSLEUHISASPTYR
11   ARGGLU

Samples:

sample_1: PYNOD PYD, [U-13C; U-15N], 0.2 mM

sample_conditions_1: ionic strength: 140 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
GB AAI04958 AAO18161 AAS67384 AIC53619 EAW68640
REF NP_789791 XP_003254944 XP_003313013 XP_003818292 XP_004050717
SP Q86W26
TPG DAA01243

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts