BMRB Entry 18244

Name: Structure of the complex of the central activation doamin of Gcn4 bound to the mediator co-activator domain 1 of Gal11/med15
Published: 2012-03-28

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PDB ID: 2lpb
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18244
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Structure of the complex of the central activation doamin of Gcn4 bound to the mediator co-activator domain 1 of Gal11/med15 PubMed: 22195967

Deposition date: 2012-02-07 Original release date: 2012-03-28

Authors: Brzovic, Peter; Heikaus, Clemens; Kisselev, Leonid; Vernon, Robert; Herbig, Eric; Pacheco, Derek; Warfield, Linda; Littlefield, Peter; Baker, David; Klevit, Rachel; Hahn, Steven

Citation: Brzovic, Peter; Heikaus, Clemens; Kisselev, Leonid; Vernon, Robert; Herbig, Eric; Pacheco, Derek; Warfield, Linda; Baker, David; Klevit, Rachel; Hahn, Steven. "The Acidic Transcription Activator Gcn4 Binds the Mediator Subunit Gal11/Med15 Using a Simple Protein Interface Forming a Fuzzy Complex" Mol. Cell 44, 942-953 (2011).

Assembly members:
entity_1, polymer, 81 residues, 9514.164 Da.
entity_2, polymer, 34 residues, 3958.110 Da.

Natural source: Common Name: baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: QRRQLTPQQQQLVNQMKVAP IPKQLLQRIPNIPPNINTWQ QVTALAQQKLLTPQDMEAAK QVYKIHQQLLFKARLQQQQA Q
entity_2: STDSTPMFEYENLEDNSKEW TSLFDNDIPVTTDD

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	487
15N chemical shifts	102
1H chemical shifts	761

Additional metadata:

Assembly
Samples and Experiments
Software
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Entity Assembly ID	Entity Name	Entity ID
1	central activation doamin of Gcn4	1
2	domain 1 of Gal11/med15	2

Samples:

sample_1: Gal11, [U-15N], 1-1.2 mM; Gcn4 2-2.4 mM; Sodum phosphate 20 mM; Sodum chloride 150 mM; PMSF 1 mM; DTT 5 mM; H20 90%; D2O 10%

sample_2: Gal11 2-2.4 mM; Gcn4, [U-15N], 1-1.2 mM; Sodum phosphate 20 mM; Sodum chloride 150 mM; PMSF 1 mM; DTT 5 mM; H20 90%; D2O 10%

sample_3: Gal11, [U-13C; U-15N], 1-1.2 mM; Gcn4 2-2.4 mM; Sodum phosphate 20 mM; Sodum chloride 150 mM; PMSF 1 mM; DTT 5 mM; H20 90%; D2O 10%

sample_4: Gal11, [U-13C; U-15N], 1-1.2 mM; Gcn4 2-2.4 mM; Sodum phosphate 20 mM; Sodum chloride 150 mM; PMSF 1 mM; DTT 5 mM; D2O 100%

sample_5: Gal11 2-2.4 mM; Gcn4, [U-13C; U-15N], 1-1.2 mM; Sodum phosphate 20 mM; Sodum chloride 150 mM; PMSF 1 mM; DTT 5 mM; H20 90%; D2O 10%

sample_conditions_1: ionic strength: 0.150 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_3	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_5	isotropic	sample_conditions_1
3D HNCA	sample_3	isotropic	sample_conditions_1
3D HNCA	sample_5	isotropic	sample_conditions_1
3D HNCO	sample_3	isotropic	sample_conditions_1
3D HNCO	sample_5	isotropic	sample_conditions_1
3D HNCACB	sample_3	isotropic	sample_conditions_1
3D HNCACB	sample_5	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_3	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_5	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_4	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_5	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_4	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_5	isotropic	sample_conditions_1
3D C(CO)NH	sample_3	isotropic	sample_conditions_1
3D C(CO)NH	sample_5	isotropic	sample_conditions_1
3D H(CCO)NH	sample_3	isotropic	sample_conditions_1
3D H(CCO)NH	sample_5	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_3	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_5	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_3	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_5	isotropic	sample_conditions_1

PDB	2LPB 2LPB
DBJ	GAA26277 GAA22815
EMBL	CAA62537 CAA99056 CAY86238 CAE52206 CAE52207 CAE52208 CAE52209 CAE52210
GB	AAA34622 AHY77262 AJP41494 AJT70913 AJT71404 AAA34640 AAA65521 AAB64486 AHY75543 EDN62959
REF	NP_014591 NP_010907
SP	P19659 P03069
TPG	DAA10733 DAA07643

Biological Magnetic Resonance Data Bank

BMRB Entry 18244

Title: Structure of the complex of the central activation doamin of Gcn4 bound to the mediator co-activator domain 1 of Gal11/med15 PubMed: 22195967

Additional metadata:

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Samples:

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