BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18248

Title: Assignments for the dually targeting peptide from Thr-tRNA synthetase   PubMed: 22863398

Deposition date: 2012-02-09 Original release date: 2012-08-27

Authors: Ye, Weihua; Spanning, Erika; Unnerstale, Sofia; Gotthold, David; Glaser, Elzbieta; Maler, Lena

Citation: Ye, Weihua; Spanning, Erika; Unnerstale, Sofia; Gotthold, David; Glaser, Elzbieta; Maler, Lena. "NMR investigations of the dual targeting peptide of Thr-tRNA synthetase and its interaction with the mitochondrial Tom20 receptor in Arabidopsisthaliana."  FEBS J. 279, 3738-3748 (2012).

Assembly members:
ThrRS-dTP(2-60), polymer, 59 residues, Formula weight is not available

Natural source:   Common Name: Thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
ThrRS-dTP(2-60): ASSHSLLFSSSFLSKPSSFT SSLRRFVYLPTRQFWPRQRH GFSTVFAVATEPAISSSGP

Data sets:
Data typeCount
13C chemical shifts49
15N chemical shifts51
1H chemical shifts277

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ThrRS-dTP1

Entities:

Entity 1, ThrRS-dTP 59 residues - Formula weight is not available

1   ALASERSERHISSERLEULEUPHESERSER
2   SERPHELEUSERLYSPROSERSERPHETHR
3   SERSERLEUARGARGPHEVALTYRLEUPRO
4   THRARGGLNPHETRPPROARGGLNARGHIS
5   GLYPHESERTHRVALPHEALAVALALATHR
6   GLUPROALAILESERSERSERGLYPRO

Samples:

sample_1: ThrRS-dTP(2-60), [U-15N], 80 – 100 uM; D2O, [U-100% 2H], 10%; sodium phosphate 50 mM

sample_2: ThrRS-dTP(2-60), [U-13C; U-15N], 80 – 100 uM; D2O, [U-100% 2H], 10%; sodium phosphate 50 mM

sample_conditions_1: pH: 6.0; temperature: 298 K

sample_conditions_2: pH: 6.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
HNCAsample_2isotropicsample_conditions_2
HN(CO)CAsample_2isotropicsample_conditions_2

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz

Related Database Links:

GB AEC05873
REF NP_671778
SP F4IFC5

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts