BMRB Entry 18261
Click here to enlarge.
PDB ID: 2lpi
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18261
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: NMR structure of a monomeric mutant (A72R) of major ampullate spidroin 1 N-terminal domain PubMed: 22706024
Deposition date: 2012-02-14 Original release date: 2012-06-26
Authors: Jaudzems, Kristaps; Nordling, Kerstin; Landreh, Michael; Rising, Anna; Askarieh, Gelareh; Knight, Stefan; Johansson, Jan
Citation: Jaudzems, Kristaps; Askarieh, Glareh; Landreh, Michael; Nordling, Kerstin; Hedhammar, My; Jornvall, Hans; Rising, Anna; Knight, Stefan; Johansson, Jan. "pH-dependent dimerization of spider silk N-terminal domain requires relocation of a wedged tryptophan side chain." J. Mol. Biol. 422, 477-487 (2012).
Assembly members:
A72R, polymer, 137 residues, 14269.917 Da.
Natural source: Common Name: Spiders Taxonomy ID: 332052 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Euprosthenops australis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
A72R: GSGNSHTTPWTNPGLAENFM
NSFMQGLSSMPGFTASQLDD
MSTIAQSMVQSIQSLAAQGR
TSPNKLQALNMRFASSMAEI
AASEEGGGSLSTKTSSIASA
MSNAFLQTTGVVNQPFINEI
TQLVSMFAQAGMNDVSA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 542 |
| 15N chemical shifts | 142 |
| 1H chemical shifts | 873 |
Additional metadata:
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts