BMRB Entry 18269
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18269
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Solution Structure of N-Terminal domain of human Conserved Dopamine Neurotrophic Factor (CDNF) PubMed: 22528768
Deposition date: 2012-02-15 Original release date: 2012-06-13
Authors: Cristiane, Latge; Katia, Cabral; Debora, Foguel; Pires, Jose Ricardo; Almeida, Marcius
Citation: Latge, Cristiane; Cabral, Katia; Almeida, Marcius; Foguel, Debora. "(1)H-, (13)C- and (15)N-NMR assignment of the N-terminal domain of human cerebral dopamine neurotrophic factor (CDNF)" Biomol. NMR Assign. ., .-. (2012).
Assembly members:
entity, polymer, 105 residues, 11965.022 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: QEAGGRPGADCEVCKEFLNR
FYKSLIDRGVNFSLDTIEKE
LISFCLDTKGKENRLCYYLG
ATKDAATKILSEVTRPMSVH
MPAMKICEKLKKLDSQICEL
KYEKT
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 360 |
| 15N chemical shifts | 98 |
| 1H chemical shifts | 688 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | N-terminal domain of human CDNF | 1 |
Entities:
Entity 1, N-terminal domain of human CDNF 105 residues - 11965.022 Da.
Mature sequence of N-terminal domain.
| 1 | GLN | GLU | ALA | GLY | GLY | ARG | PRO | GLY | ALA | ASP | ||||
| 2 | CYS | GLU | VAL | CYS | LYS | GLU | PHE | LEU | ASN | ARG | ||||
| 3 | PHE | TYR | LYS | SER | LEU | ILE | ASP | ARG | GLY | VAL | ||||
| 4 | ASN | PHE | SER | LEU | ASP | THR | ILE | GLU | LYS | GLU | ||||
| 5 | LEU | ILE | SER | PHE | CYS | LEU | ASP | THR | LYS | GLY | ||||
| 6 | LYS | GLU | ASN | ARG | LEU | CYS | TYR | TYR | LEU | GLY | ||||
| 7 | ALA | THR | LYS | ASP | ALA | ALA | THR | LYS | ILE | LEU | ||||
| 8 | SER | GLU | VAL | THR | ARG | PRO | MET | SER | VAL | HIS | ||||
| 9 | MET | PRO | ALA | MET | LYS | ILE | CYS | GLU | LYS | LEU | ||||
| 10 | LYS | LYS | LEU | ASP | SER | GLN | ILE | CYS | GLU | LEU | ||||
| 11 | LYS | TYR | GLU | LYS | THR |
Samples:
sample_1: CDNF, [U-100% 13C; U-100% 15N], 0.7 mM; D2O, [U-2H], 10%; sodium azide 5 mM; MES 20 mM; sodium chloride 150 mM; H2O 90%
sample_conditions_1: ionic strength: 175 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
ARIA, Linge, O, . - peak picking, refinement, structure solution
TOPSPIN v3, Bruker Biospin - collection, processing
CARA v1.8.4.2., Keller and Wuthrich - chemical shift assignment, data analysis
CNSSOLVE v1.1., Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure solution
NMR spectrometers:
- Bruker Avance III 800 MHz
Related Database Links:
| BMRB | 19164 |
| PDB | |
| DBJ | BAG62825 |
| GB | AAI33043 AAI33045 AIC53868 EAW86264 EHH18900 |
| REF | NP_001025125 NP_001180755 XP_002820588 XP_003257694 XP_003806807 |
| SP | Q49AH0 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts