BMRB Entry 18320
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PDB ID: 2lqm
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18320
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Title: Solution Structures of RadA intein from Pyrococcus horikoshii PubMed: 22560994
Deposition date: 2012-03-09 Original release date: 2012-05-15
Authors: Oeemig, Jesper; Zhou, Dongwen; Kajander, Tommi; Wlodawer, Alexander; Iwai, Hideo
Citation: Oeemig, Jesper; Zhou, Dongwen; Kajander, Tommi; Wlodawer, Alexander; Iwai, Hideo. "NMR and crystal structures of the Pyrococcus horikoshii RadA intein guide a strategy for engineering a highly efficient and promiscuous intein." J. Mol. Biol. 421, 85-99 (2012).
Assembly members:
PhoRadA_intein, polymer, 174 residues, 19918.725 Da.
Natural source: Common Name: euryarchaeotes Taxonomy ID: 70601 Superkingdom: Archaea Kingdom: not available Genus/species: Pyrococcus Pyrococcus horikoshii
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
PhoRadA_intein: AFARDTEVYYENDTVPHMES
IEEMYSKYASMNGELPFDNG
YAVPLDNVFVYTLDIASGEI
KKTRASYIYREKVEKLIEIK
LSSGYSLKVTPSHPVLLFRD
GLQWVPAAEVKPGDVVVGVR
EEVLRRRIISKGELEFHEVS
SVRIIDYNNWVYDLVIPETH
NFIAPNGLVLHNAQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 708 |
| 15N chemical shifts | 162 |
| 1H chemical shifts | 1204 |
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