BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18356

Title: Solution Structure of ADF like UNC-60A Protein of Caenorhabditis elegans

Deposition date: 2012-03-28 Original release date: 2013-05-20

Authors: Shukla, Vaibhav Kumar; Yadav, Rahul; Kabra, Ashish; Kumar, Dinesh; Ono, Shoichiro; Arora, Ashish

Citation: Shukla, Vaibhav Kumar; Yadav, Rahul; Jain, Anupam; Tripathi, Sarita; Kabra, Ashish; Kumar, Dinesh; Ono, Shoichiro; Arora, Ashish. "NMR Structure and Backbone dynamics of ADF like UNC-60A protein from Caenorhabditis elegans: its divergence from conventional ADF/cofilin"  J. Biol. Chem. ., .-..

Assembly members:
entity, polymer, 165 residues, 18391.953 Da.

Natural source:   Common Name: Caenorhabditis elegans   Taxonomy ID: 6239   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Caenorhabditis elegans

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: MSSGVMVDPDVQTSFQKLSE GRKEYRYIIFKIDENKVIVE AAVTQDQLGITGDDYDDSSK AAFDKFVEDVKSRTDNLTDC RYAVFDFKFTCSRVGAGTSK MDKIIFLQICPDGASIKKKM VYASSAAAIKTSLGTGKILQ FQVSDESEMSHKELLNKLGE KYGDH

Data sets:
Data typeCount
13C chemical shifts719
15N chemical shifts162
1H chemical shifts1114

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ADF like UNC-60A Protein1

Entities:

Entity 1, ADF like UNC-60A Protein 165 residues - 18391.953 Da.

1   METSERSERGLYVALMETVALASPPROASP
2   VALGLNTHRSERPHEGLNLYSLEUSERGLU
3   GLYARGLYSGLUTYRARGTYRILEILEPHE
4   LYSILEASPGLUASNLYSVALILEVALGLU
5   ALAALAVALTHRGLNASPGLNLEUGLYILE
6   THRGLYASPASPTYRASPASPSERSERLYS
7   ALAALAPHEASPLYSPHEVALGLUASPVAL
8   LYSSERARGTHRASPASNLEUTHRASPCYS
9   ARGTYRALAVALPHEASPPHELYSPHETHR
10   CYSSERARGVALGLYALAGLYTHRSERLYS
11   METASPLYSILEILEPHELEUGLNILECYS
12   PROASPGLYALASERILELYSLYSLYSMET
13   VALTYRALASERSERALAALAALAILELYS
14   THRSERLEUGLYTHRGLYLYSILELEUGLN
15   PHEGLNVALSERASPGLUSERGLUMETSER
16   HISLYSGLULEULEUASNLYSLEUGLYGLU
17   LYSTYRGLYASPHIS

Samples:

sample_1: UNC-60A, [U-99% 15N], 0.65 mM; sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 0.1%; D2O, [U-99% 2H], 7%; H2O 93%

sample_2: UNC-60A, [U-99% 13C; U-99% 15N], 0.65 mM; sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 0.1%; D2O, [U-99% 2H], 7%; H2O 93%

sample_3: UNC-60A, [U-99% 13C; U-99% 15N], 0.65 mM; sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 0.1%; D2O 100%

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D C(CO)NHsample_2isotropicsample_conditions_1
3D H(CCO)NHsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_3isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_3isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_3isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_3isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_3isotropicsample_conditions_1
2D CB(CGCDCE)HEsample_2isotropicsample_conditions_1
2D CB(CGCD)HDsample_2isotropicsample_conditions_1
3D HNCACOsample_2isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CARA, Keller and Wuthrich - data analysis

PSVS v1.4, Bhattacharya and Montelione - Validation of structural parameter

iCING, Doreleijers - Validation of structural parameter

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - Structure Calculation

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Bruker Avance3 800 MHz

Related Database Links:

PDB
EMBL CCD67021
GB AAC14458
REF NP_503425
SP Q07750

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts