BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18386

Title: Characterization of the chromoshadow domain-mediated binding of heterochromatin protein 1 alpha (HP1 alpha) to histone H3: Backbone 1H, 15N chemical shift assignments for HP1 CSDalpha(109-185)   PubMed: 22493481

Deposition date: 2012-04-10 Original release date: 2012-05-21

Authors: Richart, Alexandria; Brunner, Clair; Stott, Katherine; Murzina, Natalia; Thomas, Jean

Citation: Richart, Alexandria; Brunner, Clair; Stott, Katherine; Murzina, Natalia; Thomas, Jean. "Characterization of Chromoshadow Domain-mediated Binding of Heterochromatin Protein 1 (HP1) to Histone H3."  J. Biol. Chem. 287, 18730-18737 (2012).

Assembly members:
HP1_CSDalpha(109-185), polymer, 191 residues, Formula weight is not available

Natural source:   Common Name: Humans   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
HP1_CSDalpha(109-185): MGKKTKRTADSSSSEDEEEY VVEKVLDRRVVKGQVEYLLK WKGFSEEHNTWEPEKNLDCP ELISEFMKKYKKMKEGENNK PREKSESNKRKSNFSNSADD IKSKKKREQSNDIARGFERG LEPEKIIGATDSCGDLMFLM KWKDTDEADLVLAKEANVKC PQIVIAFYEERLTWHAYPED AENKEKETAKS

Data sets:
Data typeCount
15N chemical shifts59
1H chemical shifts61

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HP1 CSDalpha(109-185)1

Entities:

Entity 1, HP1 CSDalpha(109-185) 191 residues - Formula weight is not available

1   METGLYLYSLYSTHRLYSARGTHRALAASP
2   SERSERSERSERGLUASPGLUGLUGLUTYR
3   VALVALGLULYSVALLEUASPARGARGVAL
4   VALLYSGLYGLNVALGLUTYRLEULEULYS
5   TRPLYSGLYPHESERGLUGLUHISASNTHR
6   TRPGLUPROGLULYSASNLEUASPCYSPRO
7   GLULEUILESERGLUPHEMETLYSLYSTYR
8   LYSLYSMETLYSGLUGLYGLUASNASNLYS
9   PROARGGLULYSSERGLUSERASNLYSARG
10   LYSSERASNPHESERASNSERALAASPASP
11   ILELYSSERLYSLYSLYSARGGLUGLNSER
12   ASNASPILEALAARGGLYPHEGLUARGGLY
13   LEUGLUPROGLULYSILEILEGLYALATHR
14   ASPSERCYSGLYASPLEUMETPHELEUMET
15   LYSTRPLYSASPTHRASPGLUALAASPLEU
16   VALLEUALALYSGLUALAASNVALLYSCYS
17   PROGLNILEVALILEALAPHETYRGLUGLU
18   ARGLEUTHRTRPHISALATYRPROGLUASP
19   ALAGLUASNLYSGLULYSGLUTHRALALYS
20   SER

Samples:

sample_1: HP1_CSDalpha(109-185), [U-95% 15N], 0.5 mM; sodium phosphate 10 mM; EDTA 1 mM; DTT 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.01 M; pH: 8.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

AZARA, Boucher - processing

Analysis, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

DBJ BAB25897 BAB31173 BAC26923 BAC26966 BAC28107
EMBL CAA67960 CAG33699
GB AAA72327 AAB26994 AAC50553 AAF80993 AAH04707
REF NP_001070257 NP_001100267 NP_001103686 NP_001120793 NP_001120794
SP P45973 Q61686
TPG DAA30037 DAA30038 DAA30039 DAA30040

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts