BMRB Entry 18405

Name: Structure of Sviceucin, an type I lasso peptide from Streptomyces sviceus
Published: 2012-08-30

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PDB ID: 2ls1
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18405
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Structure of Sviceucin, an type I lasso peptide from Streptomyces sviceus

Deposition date: 2012-04-18 Original release date: 2012-08-30

Authors: Li, Yanyan; Ducasse, Remi; Blond, Alain; Zirah, Severine; Goulard, Christophe; Lescop, Ewen; Guittet, Eric; Pernodet, Jean-Luc; Rebuffat, Sylvie

Citation: Li, Yanyan; Ducasse, Remi; Blond, Alain; Zirah, Severine; Goulard, Christophe; Lescop, Ewen; Guittet, Eric; Pernodet, Jean-Luc; Rebuffat, Sylvie. "Structure and biosynthesis of Sviceucin, an type I lasso peptide from Streptomyces sviceus" . ., .-..

Assembly members:
entity, polymer, 20 residues, 2106.426 Da.

Natural source: Common Name: high GC Gram+ Taxonomy ID: 285530 Superkingdom: Bacteria Kingdom: not available Genus/species: Streptomyces sviceus

Experimental source: Production method: recombinant technology Host organism: Streptomyces coelicolor

Entity Sequences (FASTA):
entity: CVWGGDCTDFLGCGTAWICV

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	59
1H chemical shifts	119

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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1		CYS	VAL	TRP	GLY	GLY	ASP	CYS	THR	ASP	PHE
2		LEU	GLY	CYS	GLY	THR	ALA	TRP	ILE	CYS	VAL

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
2D DQF-COSY	sample_1	isotropic	sample_conditions_1

PDB	2LS1
GB	EDY58505

Biological Magnetic Resonance Data Bank

BMRB Entry 18405

Title: Structure of Sviceucin, an type I lasso peptide from Streptomyces sviceus

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1		CYS	VAL	TRP	GLY	GLY	ASP	CYS	THR	ASP	PHE
2		LEU	GLY	CYS	GLY	THR	ALA	TRP	ILE	CYS	VAL

1		CYS	VAL	TRP	GLY	GLY	ASP	CYS	THR	ASP	PHE
2		LEU	GLY	CYS	GLY	THR	ALA	TRP	ILE	CYS	VAL

1		CYS	VAL	TRP	GLY	GLY	ASP	CYS	THR	ASP	PHE
2		LEU	GLY	CYS	GLY	THR	ALA	TRP	ILE	CYS	VAL