BMRB Entry 18406
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18406
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Title: Solid-state NMR sequential assignments of Sup35NM PubMed: 23934139
Deposition date: 2012-04-19 Original release date: 2014-02-11
Authors: Schuetz, Anne; Luckgei, Nina; Habenstein, Birgit; Meier, Beat; Boeckmann, Anja
Citation: Luckgei, Nina; Schuetz, Anne; Habenstein, Birgit; Bousset, Luc; Sourigues, Yannick; Melki, Ronald; Meier, Beat; Boeckmann, Anja. "Solid-state NMR sequential assignments of the amyloid core of Sup35pNM" Biomol. NMR Assign. ., .-. (2013).
Assembly members:
Sup35NM, polymer, 273 residues, 30685.8032 Da.
Natural source: Common Name: baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Sup35NM: MGSSHHHHHHSSGLVPRGSH
MSDSNQGNNQQNYQQYSQNG
NQQQGNNRYQGYQAYNAQAQ
PAGGYYQNYQGYSGYQQGGY
QQYNPDAGYQQQYNPQGGYQ
QYNPQGGYQQQFNPQGGRGN
YKNFNYNNNLQGYQAGFQPQ
SQGMSLNDFQKQQKQAAPKP
KKTLKLVSSSGIKLANATKK
VGTKPAESDKKEEEKSAETK
EPTKEPTKVEEPVKKEEKPV
QTEEKTEEKSELPKVEDLKI
SESTHNTNNANVTSADALIK
EQEEEVDDEVVND
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 85 |
| 15N chemical shifts | 31 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Sup35NM | 1 |
Entities:
Entity 1, Sup35NM 273 residues - 30685.8032 Da.
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | HIS | ||||
| 3 | MET | SER | ASP | SER | ASN | GLN | GLY | ASN | ASN | GLN | ||||
| 4 | GLN | ASN | TYR | GLN | GLN | TYR | SER | GLN | ASN | GLY | ||||
| 5 | ASN | GLN | GLN | GLN | GLY | ASN | ASN | ARG | TYR | GLN | ||||
| 6 | GLY | TYR | GLN | ALA | TYR | ASN | ALA | GLN | ALA | GLN | ||||
| 7 | PRO | ALA | GLY | GLY | TYR | TYR | GLN | ASN | TYR | GLN | ||||
| 8 | GLY | TYR | SER | GLY | TYR | GLN | GLN | GLY | GLY | TYR | ||||
| 9 | GLN | GLN | TYR | ASN | PRO | ASP | ALA | GLY | TYR | GLN | ||||
| 10 | GLN | GLN | TYR | ASN | PRO | GLN | GLY | GLY | TYR | GLN | ||||
| 11 | GLN | TYR | ASN | PRO | GLN | GLY | GLY | TYR | GLN | GLN | ||||
| 12 | GLN | PHE | ASN | PRO | GLN | GLY | GLY | ARG | GLY | ASN | ||||
| 13 | TYR | LYS | ASN | PHE | ASN | TYR | ASN | ASN | ASN | LEU | ||||
| 14 | GLN | GLY | TYR | GLN | ALA | GLY | PHE | GLN | PRO | GLN | ||||
| 15 | SER | GLN | GLY | MET | SER | LEU | ASN | ASP | PHE | GLN | ||||
| 16 | LYS | GLN | GLN | LYS | GLN | ALA | ALA | PRO | LYS | PRO | ||||
| 17 | LYS | LYS | THR | LEU | LYS | LEU | VAL | SER | SER | SER | ||||
| 18 | GLY | ILE | LYS | LEU | ALA | ASN | ALA | THR | LYS | LYS | ||||
| 19 | VAL | GLY | THR | LYS | PRO | ALA | GLU | SER | ASP | LYS | ||||
| 20 | LYS | GLU | GLU | GLU | LYS | SER | ALA | GLU | THR | LYS | ||||
| 21 | GLU | PRO | THR | LYS | GLU | PRO | THR | LYS | VAL | GLU | ||||
| 22 | GLU | PRO | VAL | LYS | LYS | GLU | GLU | LYS | PRO | VAL | ||||
| 23 | GLN | THR | GLU | GLU | LYS | THR | GLU | GLU | LYS | SER | ||||
| 24 | GLU | LEU | PRO | LYS | VAL | GLU | ASP | LEU | LYS | ILE | ||||
| 25 | SER | GLU | SER | THR | HIS | ASN | THR | ASN | ASN | ALA | ||||
| 26 | ASN | VAL | THR | SER | ALA | ASP | ALA | LEU | ILE | LYS | ||||
| 27 | GLU | GLN | GLU | GLU | GLU | VAL | ASP | ASP | GLU | VAL | ||||
| 28 | VAL | ASN | ASP |
Samples:
Sup35NM_1: Sup35NM, [U-100% 13C; U-100% 15N], 15 ± 2 mg
Condition: ionic strength: 0 M; pH: 7.000; pressure: 1.000 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| DARR 20 ms | Sup35NM_1 | solid | Condition |
| NCA | Sup35NM_1 | solid | Condition |
| NCACB | Sup35NM_1 | solid | Condition |
| CANCO | Sup35NM_1 | solid | Condition |
| NCOCX | Sup35NM_1 | solid | Condition |
| NCACO | Sup35NM_1 | solid | Condition |
| DARR 100 ms | Sup35NM_1 | solid | Condition |
| NCOCA | Sup35NM_1 | solid | Condition |
| NCACX | Sup35NM_1 | solid | Condition |
| CCC | Sup35NM_1 | solid | Condition |
| HC INEPT | Sup35NM_1 | solid | Condition |
| NCO | Sup35NM_1 | solid | Condition |
Software:
ANALYSIS v2.1, CCPN - chemical shift assignment
CcpNMR vany, CCPN - Spectrum analysis, Spectrum display
NMR spectrometers:
- Bruker Avance 850 MHz