BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18420

Title: magainin

Deposition date: 2012-04-24 Original release date: 2013-04-01

Authors: Vermeer, Louic; Mason, James

Citation: Vermeer, Louic; Kozlowska, Justyna; Lorenz, Chris; Mason, James. "All Atom Simulations of the Initial Binding of Magainin and Pleurocidin to Membranes Comprising a Mixture of Anionic and Zwitterionic Lipids"  Not known ., .-..

Assembly members:
magainin, polymer, 23 residues, 2471.959 Da.

Natural source:   Common Name: African clawed frog   Taxonomy ID: 8355   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Xenopus laevis

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
magainin: GIGKFLHSAKKFGKAFVGEI MNS

Data sets:
Data typeCount
1H chemical shifts53

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1magainin1

Entities:

Entity 1, magainin 23 residues - 2471.959 Da.

1   GLYILEGLYLYSPHELEUHISSERALALYS
2   LYSPHEGLYLYSALAPHEVALGLYGLUILE
3   METASNSER

Samples:

sample_1: magainin 1 mM; TRIS 5 mM; TSP 0.05%; SDS, [U-2H], 100 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 105 mM; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

AQUA, Rullmann, Doreleijers and Kaptein - data analysis

SPARKY, Goddard - chemical shift assignment, peak picking

ProcheckNMR, Laskowski and MacArthur - data analysis

ARIA, Linge, O, . - structure solution

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

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