BMRB Entry 18462
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PDB ID: 2lt7
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18462
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Title: Solution NMR structure of Kaiso zinc finger DNA binding domain in complex with Kaiso binding site DNA PubMed: 22949637
Deposition date: 2012-05-15 Original release date: 2012-09-04
Authors: Buck-Koehntop, Bethany; Stanfield, Robyn; Ekiert, Damian; Martinez-Yamout, Maria; Dyson, H. Jane; Wilson, Ian; Wright, Peter
Citation: Buck-Koehntop, Bethany; Stanfield, Robyn; Ekiert, Damian; Martinez-Yamout, Maria; Dyson, H. Jane; Wilson, Ian; Wright, Peter. "Molecular basis for recognition of methylated and specific DNA sequences by the zinc finger protein Kaiso." Proc. Natl. Acad. Sci. U.S.A. 109, 15229-15234 (2012).
Assembly members:
Kaiso, polymer, 133 residues, 16086.681 Da.
DNA_strand_1, polymer, 19 residues, 5780.795 Da.
DNA_strand_2, polymer, 19 residues, 5869.857 Da.
ZINC ION, non-polymer, 65.409 Da.
Natural source: Common Name: Humans Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Kaiso: ANKRMKVKHDDHYELIVDGR
VYYICIVCKRSYVCLTSLRR
HFNIHSWEKKYPCRYCEKVF
PLAEYRTKHEIHHTGERRYQ
CLACGKSFINYQFMSSHIKS
VHSQDPSGDSKLYRLHPCRS
LQIRQYAYLSDRS
DNA_strand_1: GTGCTTCCTGCCAATAACG
DNA_strand_2: CGTTATTGGCAGGAAGCAC
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 609 |
| 15N chemical shifts | 164 |
| 1H chemical shifts | 1288 |
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