BMRB Entry 18480
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PDB ID: 2lth
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18480
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Title: NMR structure of major ampullate spidroin 1 N-terminal domain at pH 5.5 PubMed: 24510122
Deposition date: 2012-05-25 Original release date: 2013-11-26
Authors: Otikovs, Martins; Jaudzems, Kristaps; Nordling, Kerstin; Landreh, Michael; Rising, Anna; Askarieh, Glareh; Knight, Stefan; Johansson, Jan
Citation: Kronqvist, Nina; Otikovs, Martins; Chmyrov, Volodymyr; Chen, Gefei; Andersson, Marlene; Nordling, Kerstin; Landreh, Michael; Sarr, Medoune; Jornvall, Hans; Wennmalm, Stefan; Widengren, Jerker; Meng, Qing; Rising, Anna; Otzen, Daniel; Knight, Stefan; Jaudzems, Kristaps; Johansson, Jan. "Sequential pH-driven dimerization and stabilization of the N-terminal domain enables rapid spider silk formation." Nat. Commun. 5, 3254-3254 (2014).
Assembly members:
Major_ampullate_spidroin_1, polymer, 137 residues, 14183.802 Da.
Natural source: Common Name: spider Taxonomy ID: 332052 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Euprosthenops australis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Major_ampullate_spidroin_1: GSGNSHTTPWTNPGLAENFM
NSFMQGLSSMPGFTASQLDD
MSTIAQSMVQSIQSLAAQGR
TSPNKLQALNMAFASSMAEI
AASEEGGGSLSTKTSSIASA
MSNAFLQTTGVVNQPFINEI
TQLVSMFAQAGMNDVSA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 537 |
| 15N chemical shifts | 152 |
| 1H chemical shifts | 893 |
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