BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18481

Title: STRUCTURE OF LASSO PEPTIDE ASTEXIN1   PubMed: 22949633

Deposition date: 2012-05-25 Original release date: 2012-09-04

Authors: Maksimov, M.; Link, A.

Citation: Maksimov, Mikhail; Pelczer, Istvan; Link, A. James. "Precursor-centric genome-mining approach for lasso peptide discovery."  Proc. Natl. Acad. Sci. U.S.A. 109, 15223-15228 (2012).

Assembly members:
ASTEXIN1, polymer, 23 residues, 2583.721 Da.

Natural source:   Common Name: a-proteobacteria   Taxonomy ID: 78587   Superkingdom: Bacteria   Kingdom: not available   Genus/species: ASTICCACAULIS EXCENTRICUS

Experimental source:   Production method: recombinant technology   Host organism: ESCHERICHIA COLI

Entity Sequences (FASTA):
ASTEXIN1: GLSQGVEPDIGQTYFEESRI NQD

Data sets:
Data typeCount
1H chemical shifts159

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ASTEXIN11

Entities:

Entity 1, ASTEXIN1 23 residues - 2583.721 Da.

1   GLYLEUSERGLNGLYVALGLUPROASPILE
2   GLYGLNTHRTYRPHEGLUGLUSERARGILE
3   ASNGLNASP

Samples:

sample: ASTEXIN1 4.4 mg/mL

sample_conditions_1: pressure: 1 atm; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
2D TOCSYsampleisotropicsample_conditions_1
2D NOESYsampleisotropicsample_conditions_1
2D COSYsampleisotropicsample_conditions_1

Software:

CYANA_TINKER v2.1, 6.0, GUNTERT, P. (CYANA), PONDER, J. (TINKER) - refinement

TOPSPIN v2.1, Bruker Biospin - collection

MESTRENOVA v7.0.3, Mestrelab Research - data analysis

NMR spectrometers:

  • BRUKER AVANCE-III 500 MHz

Related Database Links:

BMRB 18948 25754
PDB
GB ADU13884