BMRB Entry 18527
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18527
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Title: 1H chemical shifts for the C-terminal beta-tubulin 394-455 fragment PubMed: 10211825
Deposition date: 2012-06-16 Original release date: 2012-07-13
Authors: Jimenez, M. Angeles; Andreu, Jose
Citation: Andreu, M. Angeles; Evangelio, Juan; Aranda, Carlos; Lopez-Brauet, Adamari; Andreu, David; Rico, Manuel; Lagos, Rosalba; Andreu, Jose; Monasterio, Octavio. "Helicity of alpha(404-451) and bet(394-445) tubulin C-terminal recombinant peptides" Protein Sci. 8, 788-799 (1999).
Assembly members:
beta_tubulin_394-445, polymer, 58 residues, Formula weight is not available
Natural source: Common Name: Humans Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
beta_tubulin_394-445: ARIRAPFLHWYTGEGMDEME
FTEAESNMNDLVSEYQQYQD
ATADEQGEFEEEGEEDEA
- assigned_chemical_shifts
Data type | Count |
1H chemical shifts | 340 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | beta tubulin 394-445 | 1 |
Entities:
Entity 1, beta tubulin 394-445 58 residues - Formula weight is not available
ARIRAP is the N-terminal cloning tag
1 | ALA | ARG | ILE | ARG | ALA | PRO | PHE | LEU | HIS | TRP | ||||
2 | TYR | THR | GLY | GLU | GLY | MET | ASP | GLU | MET | GLU | ||||
3 | PHE | THR | GLU | ALA | GLU | SER | ASN | MET | ASN | ASP | ||||
4 | LEU | VAL | SER | GLU | TYR | GLN | GLN | TYR | GLN | ASP | ||||
5 | ALA | THR | ALA | ASP | GLU | GLN | GLY | GLU | PHE | GLU | ||||
6 | GLU | GLU | GLY | GLU | GLU | ASP | GLU | ALA |
Samples:
sample_1: beta tubulin 394-445 2 mM; H2O 63 v/v; D2O 7 v/v; TFE, [U-99% 2H], 30 v/v; TSP 0.2 mM
sample_conditions_1: ionic strength: 0 M; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-1H COSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
xwinnmr, Bruker Biospin - processing
NMR spectrometers:
- Bruker AMX 600 MHz