BMRB Entry 18528
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18528
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Title: 1H chemical shifts for the C-terminal alfa-tubulin 404-451 fragment PubMed: 10211825
Deposition date: 2012-06-17 Original release date: 2012-07-13
Authors: Jimenez, M. Angeles; Andreu, Jose
Citation: Andreu, M. Angeles; Evangelio, Juan; Aranda, Carlos; Lopez-Brauet, Adamari; Andreu, David; Rico, Manuel; Lagos, Rosalba; Andreu, Jose; Monasterio, Octavio. "Helicity of alpha(404-451) and bet(394-445) tubulin C-terminal recombinant peptides" Protein Sci. 8, 788-799 (1999).
Assembly members:
alpha_tubulin_404-451, polymer, 48 residues, Formula weight is not available
Natural source: Common Name: Humans Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
alpha_tubulin_404-451: FVHWYVGEGMEEGEFSEARE
DMAALEKDYEEVGVDSVEGE
GEEEGEEY
- assigned_chemical_shifts
Data type | Count |
1H chemical shifts | 290 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | alpha tubulin 404-451 | 1 |
Entities:
Entity 1, alpha tubulin 404-451 48 residues - Formula weight is not available
1 | PHE | VAL | HIS | TRP | TYR | VAL | GLY | GLU | GLY | MET | ||||
2 | GLU | GLU | GLY | GLU | PHE | SER | GLU | ALA | ARG | GLU | ||||
3 | ASP | MET | ALA | ALA | LEU | GLU | LYS | ASP | TYR | GLU | ||||
4 | GLU | VAL | GLY | VAL | ASP | SER | VAL | GLU | GLY | GLU | ||||
5 | GLY | GLU | GLU | GLU | GLY | GLU | GLU | TYR |
Samples:
sample_1: alpha tubulin 404-451 2 mM; H2O 63%; D2O, [U-100% 2H], 7%; TFE, [U-99% 2H], 30 v/v; TSP 0.2 mM
sample_conditions_1: ionic strength: 0 M; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-1H COSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
xwinnmr, Bruker Biospin - processing
NMR spectrometers:
- Bruker AMX 600 MHz