BMRB Entry 18534

Name: RDC REFINED SOLUTION STRUCTURE OF DOUBLE-STRANDED RNA BINDING DOMAIN OF S. CEREVISIAE RNASE III (RNT1P) IN COMPLEX WITH THE TERMINAL RNA HAIRPIN OF SNR47 PRECURSOR
Published: 2013-02-20

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PDB ID: 2lup
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18534
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: RDC REFINED SOLUTION STRUCTURE OF DOUBLE-STRANDED RNA BINDING DOMAIN OF S. CEREVISIAE RNASE III (RNT1P) IN COMPLEX WITH THE TERMINAL RNA HAIRPIN OF SNR47 PRECURSOR PubMed: 21742266

Deposition date: 2012-06-19 Original release date: 2013-02-20

Authors: Wang, Zhonghua; Feigon, Juli

Citation: Wang, Zhonghua; Hartman, Elon; Roy, Kevin; Chanfreau, Guillaume; Feigon, Juli. "Structure of a yeast RNase III dsRBD complex with a noncanonical RNA substrate provides new insights into binding specificity of dsRBDs." Structure 19, 999-1010 (2011).

Assembly members:
Ribonuclease III, polymer, 90 residues, 9822.442 Da.
RNA (32-MER), polymer, 32 residues, 9822.442 Da.

Natural source: Common Name: baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
Ribonuclease III: GSLDMNAKRQLYSLIGYASL RLHYVTVKKPTAVDPNSIVE CRVGDGTVLGTGVGRNIKIA GIRAAENALRDKKMLDFYAK QRAAIPRSES
RNA (32-MER): GGGAUACCAUGUUCAGAAGA ACGUGGUAUCUC

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	487
15N chemical shifts	90
1H chemical shifts	740

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Entity Assembly ID	Entity Name	Entity ID
1	Ribonuclease III	1
2	RNA (32-MER)	2

1	GLY	SER	LEU	ASP	MET	ASN	ALA	LYS	ARG	GLN
2	LEU	TYR	SER	LEU	ILE	GLY	TYR	ALA	SER	LEU
3	ARG	LEU	HIS	TYR	VAL	THR	VAL	LYS	LYS	PRO
4	THR	ALA	VAL	ASP	PRO	ASN	SER	ILE	VAL	GLU
5	CYS	ARG	VAL	GLY	ASP	GLY	THR	VAL	LEU	GLY
6	THR	GLY	VAL	GLY	ARG	ASN	ILE	LYS	ILE	ALA
7	GLY	ILE	ARG	ALA	ALA	GLU	ASN	ALA	LEU	ARG
8	ASP	LYS	LYS	MET	LEU	ASP	PHE	TYR	ALA	LYS
9	GLN	ARG	ALA	ALA	ILE	PRO	ARG	SER	GLU	SER

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D HCCH-COSY	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_2	isotropic	sample_conditions_1

PDB	1T4L 1T4L 1T4N 1T4O 2LBS 2LUP 2LUQ 4OOG
BMRB	17574 18535
DBJ	GAA25690
EMBL	CAA90210 CAY82070
GB	AAB04172 AHY76694 AJP40933 AJS62105 AJS62540
REF	NP_013966
SP	Q02555
TPG	DAA10139

Biological Magnetic Resonance Data Bank

BMRB Entry 18534

Title: RDC REFINED SOLUTION STRUCTURE OF DOUBLE-STRANDED RNA BINDING DOMAIN OF S. CEREVISIAE RNASE III (RNT1P) IN COMPLEX WITH THE TERMINAL RNA HAIRPIN OF SNR47 PRECURSOR PubMed: 21742266

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	GLY	SER	LEU	ASP	MET	ASN	ALA	LYS	ARG	GLN
2	LEU	TYR	SER	LEU	ILE	GLY	TYR	ALA	SER	LEU
3	ARG	LEU	HIS	TYR	VAL	THR	VAL	LYS	LYS	PRO
4	THR	ALA	VAL	ASP	PRO	ASN	SER	ILE	VAL	GLU
5	CYS	ARG	VAL	GLY	ASP	GLY	THR	VAL	LEU	GLY
6	THR	GLY	VAL	GLY	ARG	ASN	ILE	LYS	ILE	ALA
7	GLY	ILE	ARG	ALA	ALA	GLU	ASN	ALA	LEU	ARG
8	ASP	LYS	LYS	MET	LEU	ASP	PHE	TYR	ALA	LYS
9	GLN	ARG	ALA	ALA	ILE	PRO	ARG	SER	GLU	SER

1	GLY	SER	LEU	ASP	MET	ASN	ALA	LYS	ARG	GLN
2	LEU	TYR	SER	LEU	ILE	GLY	TYR	ALA	SER	LEU
3	ARG	LEU	HIS	TYR	VAL	THR	VAL	LYS	LYS	PRO
4	THR	ALA	VAL	ASP	PRO	ASN	SER	ILE	VAL	GLU
5	CYS	ARG	VAL	GLY	ASP	GLY	THR	VAL	LEU	GLY
6	THR	GLY	VAL	GLY	ARG	ASN	ILE	LYS	ILE	ALA
7	GLY	ILE	ARG	ALA	ALA	GLU	ASN	ALA	LEU	ARG
8	ASP	LYS	LYS	MET	LEU	ASP	PHE	TYR	ALA	LYS
9	GLN	ARG	ALA	ALA	ILE	PRO	ARG	SER	GLU	SER

1	GLY	SER	LEU	ASP	MET	ASN	ALA	LYS	ARG	GLN
2	LEU	TYR	SER	LEU	ILE	GLY	TYR	ALA	SER	LEU
3	ARG	LEU	HIS	TYR	VAL	THR	VAL	LYS	LYS	PRO
4	THR	ALA	VAL	ASP	PRO	ASN	SER	ILE	VAL	GLU
5	CYS	ARG	VAL	GLY	ASP	GLY	THR	VAL	LEU	GLY
6	THR	GLY	VAL	GLY	ARG	ASN	ILE	LYS	ILE	ALA
7	GLY	ILE	ARG	ALA	ALA	GLU	ASN	ALA	LEU	ARG
8	ASP	LYS	LYS	MET	LEU	ASP	PHE	TYR	ALA	LYS
9	GLN	ARG	ALA	ALA	ILE	PRO	ARG	SER	GLU	SER