BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18542

Title: Structure and Binding Interface of the Cytosolic Tails of aXb2 Integrin   PubMed: 22844534

Deposition date: 2012-06-21 Original release date: 2012-08-23

Authors: Tan, Suet Mien; Tang, Xiao Yan; Patra, Tanala

Citation: Chua, Geok-Lin; Tang, Xiao-Yan; Patra, Alok Tanala; Tan, Suet-Mien; Bhattacharjya, Surajit. "Structure and binding interface of the cytosolic tails of X2 integrin."  PLoS ONE 7, e41924-e41924 (2012).

Assembly members:
entity, polymer, 35 residues, 3960.458 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity: KVGFFKRQYKEMMEEANGQI APENGTQTPSPPSEK

Data sets:
Data typeCount
13C chemical shifts28
1H chemical shifts163

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Cytosolic Tails of aXb2 Integrin1

Entities:

Entity 1, Cytosolic Tails of aXb2 Integrin 35 residues - 3960.458 Da.

1   LYSVALGLYPHEPHELYSARGGLNTYRLYS
2   GLUMETMETGLUGLUALAASNGLYGLNILE
3   ALAPROGLUASNGLYTHRGLNTHRPROSER
4   PROPROSERGLULYS

Samples:

MYR-ALPHA-X: DPC, [U-99% 2H], 200 mM; sodium phosphate 10 mM; D2O, [U-100% 2H], 10%; dodeclyphosphocholine 200 mM; H2O 90%; MYR-ALPHA-X 0.7 mM

MYR-ALPHA-X-D2O: DPC, [U-99% 2H], 200 mM; sodium phosphate 10 mM; D2O, [U-100% 2H], 100%; dodeclyphosphocholine 200 mM; MYR-ALPHA-X 0.7 mM

sample_conditions_1: ionic strength: 0 M; pH: 5.6; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYMYR-ALPHA-Xisotropicsample_conditions_1
2D 1H-1H NOESYMYR-ALPHA-Xisotropicsample_conditions_1
2D 1H-13C HSQCMYR-ALPHA-X-D2Oisotropicsample_conditions_1
2D 1H-1H NOESYMYR-ALPHA-X-D2Oisotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment

CYANA v2.0, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAB84873
GB AAA51620 AAA59180 AAH38237 AAX29959 ABM83412
REF NP_000878 NP_001273304 XP_002826429 XP_003280513 XP_004057604
SP P20702