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Biological Magnetic Resonance Data Bank


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BMRB Entry 18566

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18566
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Title: Telokin-like domain (TL-domain) from P22 coat protein   PubMed: 22987227

Deposition date: 2012-07-03 Original release date: 2012-11-02

Authors: Rizzo, Alessandro; Fraser, LaTasha; Sheftic, Sarah; Suhanovsky, Margaret; Teschke, Carolyn; Alexandrescu, Andrei

Citation: Rizzo, Alessandro; Fraser, LaTasha; Sheftic, Sarah; Suhanovsky, Margaret; Teschke, Carolyn; Alexandrescu, Andrei. "NMR assignments for the telokin-like domain of bacteriophage P22 coat protein."  Biomol. NMR Assignments 7, 257-260 (2013).

Assembly members:
TL-domain, polymer, 124 residues, Formula weight is not available

Natural source:   Common Name: Enterobacteria phage P22   Taxonomy ID: 10754   Superkingdom: Viruses   Kingdom: not available   Genus/species: Bacteriophage P22

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
TL-domain: GSTATGITVSGAQSFKPVAW QLDNDGNKVNVDNRFATVTL SATTGMKRGDKISFAGVKFL GQMAKNVLAQDATFSVVRVV DGTHVEITPKPVALDDVSLS PEQRAYANVNTSLADAMAVN ILNV

Data sets:
Data typeCount
13C chemical shifts511
15N chemical shifts119
1H chemical shifts804

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1TL-domain1

Entities:

Entity 1, TL-domain 124 residues - Formula weight is not available

G222 is non-native glycine that was included as part of the thrombin cleavage site. but for the sake of the numbering scheme is included here as 222

1   GLYSERTHRALATHRGLYILETHRVALSER
2   GLYALAGLNSERPHELYSPROVALALATRP
3   GLNLEUASPASNASPGLYASNLYSVALASN
4   VALASPASNARGPHEALATHRVALTHRLEU
5   SERALATHRTHRGLYMETLYSARGGLYASP
6   LYSILESERPHEALAGLYVALLYSPHELEU
7   GLYGLNMETALALYSASNVALLEUALAGLN
8   ASPALATHRPHESERVALVALARGVALVAL
9   ASPGLYTHRHISVALGLUILETHRPROLYS
10   PROVALALALEUASPASPVALSERLEUSER
11   PROGLUGLNARGALATYRALAASNVALASN
12   THRSERLEUALAASPALAMETALAVALASN
13   ILELEUASNVAL

Samples:

TL-domain-13C_15N: TL-domain, [U-13C; U-15N], 1.5 mM; sodium phosphate 20 mM; sodium azide 0.2 % w/v

TL-domain-13C_15N_in_D2O: TL-domain, [U-13C; U-15N], 1.5 mM; sodium phosphate 20 mM; sodium azide 0.2 % w/v

TL-domain-15N: TL-domain, [U-15N], 1.5 mM; sodium phosphate 20 mM; sodium azide 0.2 % w/v

TL-domain-unlabelled: TL-domain 1.5 mM; sodium phosphate 20 mM; sodium azide 0.2 % w/v

sample_condition_1: ionic strength: 20 mM; pH: 6.00; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCTL-domain-15Nisotropicsample_condition_1
2D 1H-13C HSQCTL-domain-13C_15N_in_D2Oisotropicsample_condition_1
2D DQF-COSYTL-domain-unlabelledisotropicsample_condition_1
2D DQF-TOCSYTL-domain-unlabelledisotropicsample_condition_1
3D C(CO)NHTL-domain-13C_15Nisotropicsample_condition_1
3D HNCOTL-domain-13C_15Nisotropicsample_condition_1
3D HNCATL-domain-13C_15Nisotropicsample_condition_1
3D HNCACBTL-domain-13C_15Nisotropicsample_condition_1
3D HN(CO)CATL-domain-13C_15Nisotropicsample_condition_1
3D H(CCO)NHTL-domain-13C_15Nisotropicsample_condition_1
3D HCCH-TOCSYTL-domain-13C_15N_in_D2Oisotropicsample_condition_1
3D HNHATL-domain-15Nisotropicsample_condition_1
3D 1H-15N NOESYTL-domain-15Nisotropicsample_condition_1
3D 1H-15N NOESYTL-domain-15Nisotropicsample_condition_1
3D HNHBTL-domain-15Nisotropicsample_condition_1
3D HN(CA)COTL-domain-13C_15Nisotropicsample_condition_1
2D H-H NOESY (50ms)TL-domain-unlabelledisotropicsample_condition_1
3D CCH-TOCSYTL-domain-13C_15N_in_D2Oisotropicsample_condition_1

Software:

FELIX, Accelrys Software Inc. - processing

ANALYSIS, CCPN - chemical shift assignment

VNMRJ, Varian - collection

NMR spectrometers:

  • Varian Inova 600 MHz

Related Database Links:

PDB
DBJ BAD15215 BAF80720 BAG12603 BAJ35317 BAP06070
EMBL CAR60452 CBG23391 CBY96612 CCR48948 CCT21055
GB AAA72963 AAF75047 AAL15527 AAM81389 AAX21429
REF NP_059630 NP_720329 WP_001196931 WP_001196932 WP_001196933
SP P26747
TPG DAA00987

Download simulated HSQC data in one of the following formats:
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