BMRB Entry 18623

Name: Solution structure of mutant (T170E) second CARD of human RIG-I
Published: 2012-10-19

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PDB ID: 2lwe
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18623
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Solution structure of mutant (T170E) second CARD of human RIG-I PubMed: 23063562

Deposition date: 2012-07-27 Original release date: 2012-10-19

Authors: Dutta, Kaushik

Citation: Ferrage, Fabien; Dutta, Kaushik; Nistal-Villan, Estanislao; Patel, Jenish; Sanchez-Aparicio, Maria; De Ioannes, Pablo; Buku, Angeliki; Aseguinolaza, Gloria Gonzalez; Garcia-Sastre, Adolfo; Aggarwal, Aneel. "Structure and dynamics of the second CARD of human RIG-I provide mechanistic insights into regulation of RIG-I activation." Structure 20, 2048-2061 (2012).

Assembly members:
mutant (T170E) second CARD of human RIG-I, polymer, 100 residues, 11397.522 Da.

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
mutant (T170E) second CARD of human RIG-I: GSHMKKIEKLEEYRLLLKRL QPEFKTRIIPTDIISDLSEC LINQECEEILQICSTKGMMA GAEKLVECLLRSDKENWPKE LKLALEKERNKFSELWIVEK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	431
15N chemical shifts	96
1H chemical shifts	679

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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1	GLY	SER	HIS	MET	LYS	LYS	ILE	GLU	LYS	LEU
2	GLU	GLU	TYR	ARG	LEU	LEU	LEU	LYS	ARG	LEU
3	GLN	PRO	GLU	PHE	LYS	THR	ARG	ILE	ILE	PRO
4	THR	ASP	ILE	ILE	SER	ASP	LEU	SER	GLU	CYS
5	LEU	ILE	ASN	GLN	GLU	CYS	GLU	GLU	ILE	LEU
6	GLN	ILE	CYS	SER	THR	LYS	GLY	MET	MET	ALA
7	GLY	ALA	GLU	LYS	LEU	VAL	GLU	CYS	LEU	LEU
8	ARG	SER	ASP	LYS	GLU	ASN	TRP	PRO	LYS	GLU
9	LEU	LYS	LEU	ALA	LEU	GLU	LYS	GLU	ARG	ASN
10	LYS	PHE	SER	GLU	LEU	TRP	ILE	VAL	GLU	LYS

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_2	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_2	isotropic	sample_conditions_1
3D C(CO)NH	sample_2	isotropic	sample_conditions_1
3D H(CCO)NH	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_3	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_3	isotropic	sample_conditions_1

BMRB	18622
PDB	2LWD 2LWE 4NQK 4P4H
DBJ	BAG11006 BAG54273
EMBL	CAI46068
GB	AAD19826 AAI07732 AAI32787 AAI36611 EAW58547
REF	NP_055129 XP_001156662 XP_002819761 XP_003830148 XP_004047964
SP	O95786

Biological Magnetic Resonance Data Bank

BMRB Entry 18623

Title: Solution structure of mutant (T170E) second CARD of human RIG-I PubMed: 23063562

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	GLY	SER	HIS	MET	LYS	LYS	ILE	GLU	LYS	LEU
2	GLU	GLU	TYR	ARG	LEU	LEU	LEU	LYS	ARG	LEU
3	GLN	PRO	GLU	PHE	LYS	THR	ARG	ILE	ILE	PRO
4	THR	ASP	ILE	ILE	SER	ASP	LEU	SER	GLU	CYS
5	LEU	ILE	ASN	GLN	GLU	CYS	GLU	GLU	ILE	LEU
6	GLN	ILE	CYS	SER	THR	LYS	GLY	MET	MET	ALA
7	GLY	ALA	GLU	LYS	LEU	VAL	GLU	CYS	LEU	LEU
8	ARG	SER	ASP	LYS	GLU	ASN	TRP	PRO	LYS	GLU
9	LEU	LYS	LEU	ALA	LEU	GLU	LYS	GLU	ARG	ASN
10	LYS	PHE	SER	GLU	LEU	TRP	ILE	VAL	GLU	LYS

1	GLY	SER	HIS	MET	LYS	LYS	ILE	GLU	LYS	LEU
2	GLU	GLU	TYR	ARG	LEU	LEU	LEU	LYS	ARG	LEU
3	GLN	PRO	GLU	PHE	LYS	THR	ARG	ILE	ILE	PRO
4	THR	ASP	ILE	ILE	SER	ASP	LEU	SER	GLU	CYS
5	LEU	ILE	ASN	GLN	GLU	CYS	GLU	GLU	ILE	LEU
6	GLN	ILE	CYS	SER	THR	LYS	GLY	MET	MET	ALA
7	GLY	ALA	GLU	LYS	LEU	VAL	GLU	CYS	LEU	LEU
8	ARG	SER	ASP	LYS	GLU	ASN	TRP	PRO	LYS	GLU
9	LEU	LYS	LEU	ALA	LEU	GLU	LYS	GLU	ARG	ASN
10	LYS	PHE	SER	GLU	LEU	TRP	ILE	VAL	GLU	LYS

1	GLY	SER	HIS	MET	LYS	LYS	ILE	GLU	LYS	LEU
2	GLU	GLU	TYR	ARG	LEU	LEU	LEU	LYS	ARG	LEU
3	GLN	PRO	GLU	PHE	LYS	THR	ARG	ILE	ILE	PRO
4	THR	ASP	ILE	ILE	SER	ASP	LEU	SER	GLU	CYS
5	LEU	ILE	ASN	GLN	GLU	CYS	GLU	GLU	ILE	LEU
6	GLN	ILE	CYS	SER	THR	LYS	GLY	MET	MET	ALA
7	GLY	ALA	GLU	LYS	LEU	VAL	GLU	CYS	LEU	LEU
8	ARG	SER	ASP	LYS	GLU	ASN	TRP	PRO	LYS	GLU
9	LEU	LYS	LEU	ALA	LEU	GLU	LYS	GLU	ARG	ASN
10	LYS	PHE	SER	GLU	LEU	TRP	ILE	VAL	GLU	LYS