BMRB Entry 18692
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18692
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Title: Solution structure of HP1264 from Helicobacter pylori PubMed: 23406339
Deposition date: 2012-08-31 Original release date: 2012-10-01
Authors: Lee, Ki-Young
Citation: Lee, Ki-Young; Kim, Ji-Hun; Lee, Kyu-Yeon; Lee, Jiyun; Lee, Ingyun; Bae, Ye-Ji; Lee, Bong-Jin. "Structural characterization of HP1264 reveals a novel fold for the flavin mononucleotide binding protein." Biochemistry 52, 1583-1593 (2013).
Assembly members:
entity, polymer, 76 residues, 8939.449 Da.
Natural source: Common Name: Helicobacter pylori Taxonomy ID: 210 Superkingdom: Bacteria Kingdom: not available Genus/species: Helicobacter pylori
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: MKRFDLRPLKAGIFERLEEL
IEKEMQPNEVAIFMFEVGDF
SNIPKSAEFIQSKGHELLNS
LRFNQADWTIVVRKKA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 301 |
| 15N chemical shifts | 71 |
| 1H chemical shifts | 507 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HP1264 | 1 |
Entities:
Entity 1, HP1264 76 residues - 8939.449 Da.
| 1 | MET | LYS | ARG | PHE | ASP | LEU | ARG | PRO | LEU | LYS | ||||
| 2 | ALA | GLY | ILE | PHE | GLU | ARG | LEU | GLU | GLU | LEU | ||||
| 3 | ILE | GLU | LYS | GLU | MET | GLN | PRO | ASN | GLU | VAL | ||||
| 4 | ALA | ILE | PHE | MET | PHE | GLU | VAL | GLY | ASP | PHE | ||||
| 5 | SER | ASN | ILE | PRO | LYS | SER | ALA | GLU | PHE | ILE | ||||
| 6 | GLN | SER | LYS | GLY | HIS | GLU | LEU | LEU | ASN | SER | ||||
| 7 | LEU | ARG | PHE | ASN | GLN | ALA | ASP | TRP | THR | ILE | ||||
| 8 | VAL | VAL | ARG | LYS | LYS | ALA |
Samples:
sample_1: HP1264, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 150 mM
sample_conditions_1: ionic strength: 0.15 M; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAJ55796 BAJ56230 BAJ58776 BAJ60297 BAM97127 |
| EMBL | CAX29950 CBI65773 |
| GB | AAD06771 AAD08321 ABF85275 ACD48710 ACI27958 |
| REF | NP_208056 WP_000819159 WP_000819160 WP_000819162 WP_000819163 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts