BMRB Entry 18803
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18803
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Title: SR-linker peptide PubMed: 23302895
Deposition date: 2012-10-22 Original release date: 2013-01-29
Authors: Keane, Sarah; Giedroc, David
Citation: Keane, Sarah; Giedroc, David. "Solution Structure of Mouse Hepatitis Virus (MHV) nsp3a and Determinants of the Interaction with MHV Nucleocapsid (N) Protein." J. Virol. 87, 3502-3515 (2013).
Assembly members:
SR-linker_peptide, polymer, 34 residues, Formula weight is not available
Natural source: Common Name: Murine Hepatitis Virus Taxonomy ID: 11142 Superkingdom: Viruses Kingdom: not available Genus/species: Betacoronavirus Murine coronavirus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
SR-linker_peptide: CAPASRSGSRSQSRGPNNRA
RSSSNQRQPASTYK
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 60 |
15N chemical shifts | 27 |
1H chemical shifts | 27 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | MHV n-linker peptide | 1 |
Entities:
Entity 1, MHV n-linker peptide 34 residues - Formula weight is not available
1 | CYS | ALA | PRO | ALA | SER | ARG | SER | GLY | SER | ARG | ||||
2 | SER | GLN | SER | ARG | GLY | PRO | ASN | ASN | ARG | ALA | ||||
3 | ARG | SER | SER | SER | ASN | GLN | ARG | GLN | PRO | ALA | ||||
4 | SER | THR | TYR | LYS |
Samples:
sample_1: SR-linker peptide, [U-100% 13C; U-100% 15N], 1.8 mM; potassium phosphate 50 mM; potassium chloride 100 mM; TCEP 5 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 150 mM; pH: 6.0; pressure: 1 atm; temperature: 273 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
BEST 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
BEST 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
BEST 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
BEST 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
BEST 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis
SPARKY, Goddard - data analysis, peak picking
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
DBJ | BAA01591 BAJ04701 |
EMBL | CAA25198 |
GB | AAA46439 AAA46444 AAA46447 AAA46462 AAA46468 |
REF | NP_045302 YP_003029852 |
SP | P03416 P18446 P18447 P18448 Q02915 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts