BMRB Entry 18806
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18806
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Title: Solution Structure of Miz-1 zinc fingers 5 to 7 PubMed: 23975355
Deposition date: 2012-10-24 Original release date: 2012-12-07
Authors: Bernard, David; Bedard, Mikael; Bilodeau, Josee; Lavigne, Pierre
Citation: Bernard, David; Bedard, Mikael; Bilodeau, Josee; Lavigne, Pierre. "Structural and dynamical characterization of the Miz-1 zinc fingers 5-8 by solution-state NMR." J. Biomol. NMR 57, 103-116 (2013).
Assembly members:
Miz58, polymer, 112 residues, 12953.9494 Da.
ZINC ION, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Miz58: MKPYQCDYCGRSFSDPTSKM
RHLETHDTDKEHKCPHCDKK
FNQVGNLKAHLKIHIADGPL
KCRECGKQFTTSGNLKRHLR
IHSGEKPYVCIHCQRQFADP
GALQRHVRIHTG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 429 |
| 15N chemical shifts | 98 |
| 1H chemical shifts | 659 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Miz58 | 1 |
| 2 | Zinc ions | 2 |
Entities:
Entity 1, Miz58 112 residues - 12953.9494 Da.
| 1 | MET | LYS | PRO | TYR | GLN | CYS | ASP | TYR | CYS | GLY | ||||
| 2 | ARG | SER | PHE | SER | ASP | PRO | THR | SER | LYS | MET | ||||
| 3 | ARG | HIS | LEU | GLU | THR | HIS | ASP | THR | ASP | LYS | ||||
| 4 | GLU | HIS | LYS | CYS | PRO | HIS | CYS | ASP | LYS | LYS | ||||
| 5 | PHE | ASN | GLN | VAL | GLY | ASN | LEU | LYS | ALA | HIS | ||||
| 6 | LEU | LYS | ILE | HIS | ILE | ALA | ASP | GLY | PRO | LEU | ||||
| 7 | LYS | CYS | ARG | GLU | CYS | GLY | LYS | GLN | PHE | THR | ||||
| 8 | THR | SER | GLY | ASN | LEU | LYS | ARG | HIS | LEU | ARG | ||||
| 9 | ILE | HIS | SER | GLY | GLU | LYS | PRO | TYR | VAL | CYS | ||||
| 10 | ILE | HIS | CYS | GLN | ARG | GLN | PHE | ALA | ASP | PRO | ||||
| 11 | GLY | ALA | LEU | GLN | ARG | HIS | VAL | ARG | ILE | HIS | ||||
| 12 | THR | GLY |
Entity 2, Zinc ions - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: Miz58, [U-13C; U-15N], 0.65 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.05 M; pH: 7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC/HMQC | sample_1 | isotropic | sample_conditions_1 |
| Expt_5 (H[N[co[{CA|ca[C]}]]]) | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC/HMQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
ARIA v2.2, Nilges, Rieping, Habeck, Bardiaux, Bernard and Malliavin - structure calculations, refinement
CNS v1.21, Brunger, Adams, Clore, Gros, Nilges and Read - structure calculations, refinement
ANALYSIS v2.1, CCPN - chemical shift assignment, data analysis
DANGLE v1.1, CCPN - data analysis
NMRPipe v7.4, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Varian INOVA 600 MHz
Download simulated HSQC data in one of the following formats:
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or all simulated shifts
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or all simulated shifts