BMRB Entry 18853

Name: Backbone and side-chain 1H, 13C, 15N NMR assignment of the N-terminal domain of Escherichia coli LpoA
Published: 2014-02-14

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PDB ID: 2mhk
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18853
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Backbone and side-chain 1H, 13C, 15N NMR assignment of the N-terminal domain of Escherichia coli LpoA PubMed: 24493340

Deposition date: 2012-11-23 Original release date: 2014-02-14

Authors: Jean, Nicolas; Bougault, Catherine; Derouaux, Adeline; Callens, Gilles; Vollmer, Waldemar; Simorre, Jean-Pierre

Citation: Jean, Nicolas; Bougault, Catherine; Derouaux, Adeline; Callens, Gilles; Vollmer, Waldemar; Simorre, Jean-Pierre. "Backbone and side-chain (1)H, (13)C, and (15)N NMR assignments of the N-terminal domain of Escherichia coli LpoA." Biomol. NMR Assignments ., .-. (2014).

Assembly members:
LpoA_n-ter, polymer, 250 residues, 28020.5087 Da.

Natural source: Common Name: Escherichia coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
LpoA_n-ter: MGSSHHHHHHSSGLVPRGSH MGTHTPDQSTAYMQGTAQAD SAFYLQQMQQSSDDTRINWQ LLAIRALVKEGKTGQAVELF NQLPQELNDAQRREKTLLAV EIKLAQKDFAGAQNLLAKIT PADLEQNQQARYWQAKIDAS QGRPSIDLLRALIAQEPLLG AKEKQQNIDATWQALSSMTQ EQANTLVINADENILQGWLD LQRVWFDNRNDPDMMKAGIA DWQKRYPNNPGAKMLPTQLV NVKAFKPAST

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list

Data type	Count
13C chemical shifts	1036
15N chemical shifts	265
1H chemical shifts	1618

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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1	MET	GLY	SER	SER	HIS	HIS	HIS	HIS	HIS	HIS
2	SER	SER	GLY	LEU	VAL	PRO	ARG	GLY	SER	HIS
3	MET	GLY	THR	HIS	THR	PRO	ASP	GLN	SER	THR
4	ALA	TYR	MET	GLN	GLY	THR	ALA	GLN	ALA	ASP
5	SER	ALA	PHE	TYR	LEU	GLN	GLN	MET	GLN	GLN
6	SER	SER	ASP	ASP	THR	ARG	ILE	ASN	TRP	GLN
7	LEU	LEU	ALA	ILE	ARG	ALA	LEU	VAL	LYS	GLU
8	GLY	LYS	THR	GLY	GLN	ALA	VAL	GLU	LEU	PHE
9	ASN	GLN	LEU	PRO	GLN	GLU	LEU	ASN	ASP	ALA
10	GLN	ARG	ARG	GLU	LYS	THR	LEU	LEU	ALA	VAL
11	GLU	ILE	LYS	LEU	ALA	GLN	LYS	ASP	PHE	ALA
12	GLY	ALA	GLN	ASN	LEU	LEU	ALA	LYS	ILE	THR
13	PRO	ALA	ASP	LEU	GLU	GLN	ASN	GLN	GLN	ALA
14	ARG	TYR	TRP	GLN	ALA	LYS	ILE	ASP	ALA	SER
15	GLN	GLY	ARG	PRO	SER	ILE	ASP	LEU	LEU	ARG
16	ALA	LEU	ILE	ALA	GLN	GLU	PRO	LEU	LEU	GLY
17	ALA	LYS	GLU	LYS	GLN	GLN	ASN	ILE	ASP	ALA
18	THR	TRP	GLN	ALA	LEU	SER	SER	MET	THR	GLN
19	GLU	GLN	ALA	ASN	THR	LEU	VAL	ILE	ASN	ALA
20	ASP	GLU	ASN	ILE	LEU	GLN	GLY	TRP	LEU	ASP
21	LEU	GLN	ARG	VAL	TRP	PHE	ASP	ASN	ARG	ASN
22	ASP	PRO	ASP	MET	MET	LYS	ALA	GLY	ILE	ALA
23	ASP	TRP	GLN	LYS	ARG	TYR	PRO	ASN	ASN	PRO
24	GLY	ALA	LYS	MET	LEU	PRO	THR	GLN	LEU	VAL
25	ASN	VAL	LYS	ALA	PHE	LYS	PRO	ALA	SER	THR

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC/HMQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
H[N[co[{CA\|ca[C]}]]]	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
H[N[ca[CO]]]	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
HccoNH	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC/HMQC aliphatic	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC/HMQC aromatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

UniProt	P45464
PDB	2MHK
DBJ	BAB37451 BAE77193 BAG78957 BAI27426 BAI32606
EMBL	CAP77610 CAQ33482 CAR00111 CAR04759 CAR09810
GB	AAA57950 AAC76181 AAG58283 AAN44655 AAN82341
REF	NP_312055 NP_417616 NP_708948 WP_000112978 WP_000249084
SP	D2A869 P45464 Q0T0D4 Q3YX94 Q8FDA1

Biological Magnetic Resonance Data Bank

BMRB Entry 18853

Title: Backbone and side-chain 1H, 13C, 15N NMR assignment of the N-terminal domain of Escherichia coli LpoA PubMed: 24493340

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	MET	GLY	SER	SER	HIS	HIS	HIS	HIS	HIS	HIS
2	SER	SER	GLY	LEU	VAL	PRO	ARG	GLY	SER	HIS
3	MET	GLY	THR	HIS	THR	PRO	ASP	GLN	SER	THR
4	ALA	TYR	MET	GLN	GLY	THR	ALA	GLN	ALA	ASP
5	SER	ALA	PHE	TYR	LEU	GLN	GLN	MET	GLN	GLN
6	SER	SER	ASP	ASP	THR	ARG	ILE	ASN	TRP	GLN
7	LEU	LEU	ALA	ILE	ARG	ALA	LEU	VAL	LYS	GLU
8	GLY	LYS	THR	GLY	GLN	ALA	VAL	GLU	LEU	PHE
9	ASN	GLN	LEU	PRO	GLN	GLU	LEU	ASN	ASP	ALA
10	GLN	ARG	ARG	GLU	LYS	THR	LEU	LEU	ALA	VAL
11	GLU	ILE	LYS	LEU	ALA	GLN	LYS	ASP	PHE	ALA
12	GLY	ALA	GLN	ASN	LEU	LEU	ALA	LYS	ILE	THR
13	PRO	ALA	ASP	LEU	GLU	GLN	ASN	GLN	GLN	ALA
14	ARG	TYR	TRP	GLN	ALA	LYS	ILE	ASP	ALA	SER
15	GLN	GLY	ARG	PRO	SER	ILE	ASP	LEU	LEU	ARG
16	ALA	LEU	ILE	ALA	GLN	GLU	PRO	LEU	LEU	GLY
17	ALA	LYS	GLU	LYS	GLN	GLN	ASN	ILE	ASP	ALA
18	THR	TRP	GLN	ALA	LEU	SER	SER	MET	THR	GLN
19	GLU	GLN	ALA	ASN	THR	LEU	VAL	ILE	ASN	ALA
20	ASP	GLU	ASN	ILE	LEU	GLN	GLY	TRP	LEU	ASP
21	LEU	GLN	ARG	VAL	TRP	PHE	ASP	ASN	ARG	ASN
22	ASP	PRO	ASP	MET	MET	LYS	ALA	GLY	ILE	ALA
23	ASP	TRP	GLN	LYS	ARG	TYR	PRO	ASN	ASN	PRO
24	GLY	ALA	LYS	MET	LEU	PRO	THR	GLN	LEU	VAL
25	ASN	VAL	LYS	ALA	PHE	LYS	PRO	ALA	SER	THR

1	MET	GLY	SER	SER	HIS	HIS	HIS	HIS	HIS	HIS
2	SER	SER	GLY	LEU	VAL	PRO	ARG	GLY	SER	HIS
3	MET	GLY	THR	HIS	THR	PRO	ASP	GLN	SER	THR
4	ALA	TYR	MET	GLN	GLY	THR	ALA	GLN	ALA	ASP
5	SER	ALA	PHE	TYR	LEU	GLN	GLN	MET	GLN	GLN
6	SER	SER	ASP	ASP	THR	ARG	ILE	ASN	TRP	GLN
7	LEU	LEU	ALA	ILE	ARG	ALA	LEU	VAL	LYS	GLU
8	GLY	LYS	THR	GLY	GLN	ALA	VAL	GLU	LEU	PHE
9	ASN	GLN	LEU	PRO	GLN	GLU	LEU	ASN	ASP	ALA
10	GLN	ARG	ARG	GLU	LYS	THR	LEU	LEU	ALA	VAL
11	GLU	ILE	LYS	LEU	ALA	GLN	LYS	ASP	PHE	ALA
12	GLY	ALA	GLN	ASN	LEU	LEU	ALA	LYS	ILE	THR
13	PRO	ALA	ASP	LEU	GLU	GLN	ASN	GLN	GLN	ALA
14	ARG	TYR	TRP	GLN	ALA	LYS	ILE	ASP	ALA	SER
15	GLN	GLY	ARG	PRO	SER	ILE	ASP	LEU	LEU	ARG
16	ALA	LEU	ILE	ALA	GLN	GLU	PRO	LEU	LEU	GLY
17	ALA	LYS	GLU	LYS	GLN	GLN	ASN	ILE	ASP	ALA
18	THR	TRP	GLN	ALA	LEU	SER	SER	MET	THR	GLN
19	GLU	GLN	ALA	ASN	THR	LEU	VAL	ILE	ASN	ALA
20	ASP	GLU	ASN	ILE	LEU	GLN	GLY	TRP	LEU	ASP
21	LEU	GLN	ARG	VAL	TRP	PHE	ASP	ASN	ARG	ASN
22	ASP	PRO	ASP	MET	MET	LYS	ALA	GLY	ILE	ALA
23	ASP	TRP	GLN	LYS	ARG	TYR	PRO	ASN	ASN	PRO
24	GLY	ALA	LYS	MET	LEU	PRO	THR	GLN	LEU	VAL
25	ASN	VAL	LYS	ALA	PHE	LYS	PRO	ALA	SER	THR

1	MET	GLY	SER	SER	HIS	HIS	HIS	HIS	HIS	HIS
2	SER	SER	GLY	LEU	VAL	PRO	ARG	GLY	SER	HIS
3	MET	GLY	THR	HIS	THR	PRO	ASP	GLN	SER	THR
4	ALA	TYR	MET	GLN	GLY	THR	ALA	GLN	ALA	ASP
5	SER	ALA	PHE	TYR	LEU	GLN	GLN	MET	GLN	GLN
6	SER	SER	ASP	ASP	THR	ARG	ILE	ASN	TRP	GLN
7	LEU	LEU	ALA	ILE	ARG	ALA	LEU	VAL	LYS	GLU
8	GLY	LYS	THR	GLY	GLN	ALA	VAL	GLU	LEU	PHE
9	ASN	GLN	LEU	PRO	GLN	GLU	LEU	ASN	ASP	ALA
10	GLN	ARG	ARG	GLU	LYS	THR	LEU	LEU	ALA	VAL
11	GLU	ILE	LYS	LEU	ALA	GLN	LYS	ASP	PHE	ALA
12	GLY	ALA	GLN	ASN	LEU	LEU	ALA	LYS	ILE	THR
13	PRO	ALA	ASP	LEU	GLU	GLN	ASN	GLN	GLN	ALA
14	ARG	TYR	TRP	GLN	ALA	LYS	ILE	ASP	ALA	SER
15	GLN	GLY	ARG	PRO	SER	ILE	ASP	LEU	LEU	ARG
16	ALA	LEU	ILE	ALA	GLN	GLU	PRO	LEU	LEU	GLY
17	ALA	LYS	GLU	LYS	GLN	GLN	ASN	ILE	ASP	ALA
18	THR	TRP	GLN	ALA	LEU	SER	SER	MET	THR	GLN
19	GLU	GLN	ALA	ASN	THR	LEU	VAL	ILE	ASN	ALA
20	ASP	GLU	ASN	ILE	LEU	GLN	GLY	TRP	LEU	ASP
21	LEU	GLN	ARG	VAL	TRP	PHE	ASP	ASN	ARG	ASN
22	ASP	PRO	ASP	MET	MET	LYS	ALA	GLY	ILE	ALA
23	ASP	TRP	GLN	LYS	ARG	TYR	PRO	ASN	ASN	PRO
24	GLY	ALA	LYS	MET	LEU	PRO	THR	GLN	LEU	VAL
25	ASN	VAL	LYS	ALA	PHE	LYS	PRO	ALA	SER	THR