BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18865

Title: Ovine Doppel Signal peptide (1-30)   PubMed: 23973967

Deposition date: 2012-11-28 Original release date: 2013-10-14

Authors: Pimenta, Jorge; Viegas, Aldino; Sardinha, Joao; Santos, Ana; Cantante, Catia; Dias, Fernando; Soares, Rui; Cabrita, Eurico; Fontes, Carlos; Prates, Jose; Pereira, Rosa

Citation: Pimenta, Jorge; Viegas, Aldino; Sardinha, Joao; Martins, Ivo; Cabrita, Eurico; Fontes, Carlos; Prates, Jose; Pereira, Rosa. "NMR solution structure and SRP54M predicted interaction of the N-terminal sequence (1-30) of the ovine Doppel protein"  Peptides 49, 32-40 (2013).

Assembly members:
OvDpl(1-30), polymer, 30 residues, 3321.144 Da.

Natural source:   Common Name: sheep   Taxonomy ID: 9940   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Ovis aries

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
OvDpl(1-30): MRKHLGGCWLAIVCVLLFSQ LSSVKARGIK

Data sets:
Data typeCount
1H chemical shifts226

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1OvDpl(1-30)1

Entities:

Entity 1, OvDpl(1-30) 30 residues - 3321.144 Da.

1   METARGLYSHISLEUGLYGLYCYSTRPLEU
2   ALAILEVALCYSVALLEULEUPHESERGLN
3   LEUSERSERVALLYSALAARGGLYILELYS

Samples:

sample_1: DHPC 100 mM; OvDpl(1-30) 3 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 3.5; pressure: 1 atm; temperature: 318 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

AMBER v12, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

SPARKY, Goddard - chemical shift assignment

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
EMBL CAC22340
GB AAK08630 AAK77164 AAO44923 ABN79633 ABN79636
REF NP_001009261 XP_004276488 XP_004324935 XP_005688215 XP_005974367
SP Q9GJY2
TPE CAL59571