BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18887

Title: Solution structure of hypothetical protein lmo0427

Deposition date: 2012-12-10 Original release date: 2012-12-21

Authors: Zhang, Yongbo; Winsor, James; Radhakrishnan, Ishwar; Anderson, Wayne

Citation: Zhang, Yongbo; Winsor, James; Radhakrishnan, Ishwar; Anderson, Wayne. "Solution structure of hypothetical protein lmo0427"  Not known ., .-..

Assembly members:
entity, polymer, 106 residues, 11395.479 Da.

Natural source:   Common Name: Staphylococcus aureus   Taxonomy ID: 1280   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: MKRKIIAVTACATGVAHTYM AAQALKKGAKKMGNLIKVET QGATGIENELTEKDVNIGEV VIFAVDTKVRNKERFDGKVV LEVPVSAPIKDAEKVINAAL ALIDEK

Data sets:
Data typeCount
13C chemical shifts462
15N chemical shifts112
1H chemical shifts766

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hypothetical protein lmo04271

Entities:

Entity 1, hypothetical protein lmo0427 106 residues - 11395.479 Da.

1   METLYSARGLYSILEILEALAVALTHRALA
2   CYSALATHRGLYVALALAHISTHRTYRMET
3   ALAALAGLNALALEULYSLYSGLYALALYS
4   LYSMETGLYASNLEUILELYSVALGLUTHR
5   GLNGLYALATHRGLYILEGLUASNGLULEU
6   THRGLULYSASPVALASNILEGLYGLUVAL
7   VALILEPHEALAVALASPTHRLYSVALARG
8   ASNLYSGLUARGPHEASPGLYLYSVALVAL
9   LEUGLUVALPROVALSERALAPROILELYS
10   ASPALAGLULYSVALILEASNALAALALEU
11   ALALEUILEASPGLULYS

Samples:

sample_1: Listeria monocytogenes EGD-e, [U-95% 13C; U-95% 15N], 1.2 mM; potassium phosphate 20 mM; sodium chloride 100 mM

sample_2: Listeria monocytogenes EGD-e, [U-95% 13C; U-95% 15N], 1.2 mM; potassium phosphate 20 mM; sodium chloride 100 mM

sample_conditions_1: ionic strength: 0.2 M; pH: 6.7; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D HCCH-COSYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1

Software:

ARIA v1.2, Linge, O, . - refinement, structure solution

NMRPipe, Delaglio, Zhengrong and Bax - processing

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
EMBL CAC98506 CAK19805 CAR83139 CAS04216 CBY02952
GB AAT03227 ACK40550 ADB67216 ADB70305 AEH91456
REF NP_463956 WP_003722977 WP_003760426 WP_036088242 WP_047584151

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts