BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18926

Title: Solution structure of MCh-1: A novel inhibitor cystine knot peptide from Momordica charantia   PubMed: 24116036

Deposition date: 2013-01-01 Original release date: 2013-10-14

Authors: HE, WEN-JUN; CHAN, LAI YUE; CLARK, RICHARD; TANG, JUN; ZENG, GUANG-ZHI; FRANCO, OCTAVIO; CANTACESSI, CINZIA; CRAIK, DAVID; DALY, NORELLE; TAN, NING-HUA

Citation: HE, WEN-JUN; CHAN, LAI YUE; CLARK, RICHARD; TANG, JUN; ZENG, GUANG-ZHI; FRANCO, OCTAVIO; CANTACESSI, CINZIA; CRAIK, DAVID; DALY, NORELLE; TAN, NING-HUA. "Novel Inhibitor Cystine Knot Peptides from Momordica charantia"  PLoS One 8, e75334-e75334 (2013).

Assembly members:
MCh-1, polymer, 32 residues, 3030.585 Da.

Natural source:   Common Name: Eudicots   Taxonomy ID: 3673   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Momordica charantia

Experimental source:   Production method: purified from the natural source   Host organism: Momordica charantia

Entity Sequences (FASTA):
MCh-1: GCAGKSCNILGSDPCDAGCF CLPVGIVAGVCV

Data sets:
Data typeCount
1H chemical shifts178

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1MCh-11

Entities:

Entity 1, MCh-1 32 residues - 3030.585 Da.

1   GLYCYSALAGLYLYSSERCYSASNILELEU
2   GLYSERASPPROCYSASPALAGLYCYSPHE
3   CYSLEUPROVALGLYILEVALALAGLYVAL
4   CYSVAL

Samples:

sample_1: MCh-1 3 ± 0.2 mM; H2O 90%; D2O 10%; acetonitrile 27%

sample_conditions_1: pH: 5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMR spectrometers:

  • Bruker ARX 600 MHz

Related Database Links:

PDB